Modification of proteins with cyclodextrins prevents aggregation and surface adsorption and increases thermal stability

Deepali Prashar; Dawei Cui; Debjyoti Bandyopadhyay; Yan Yeung Luk

doi:10.1021/la203271u

Modification of proteins with cyclodextrins prevents aggregation and surface adsorption and increases thermal stability

Deepali Prashar, Dawei Cui, Debjyoti Bandyopadhyay, Yan Yeung Luk

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

This work describes a general approach for preventing protein aggregation and surface adsorption by modifying proteins with β-cyclodextrins (βCD) via an efficient water-driven ligation. As compared to native unmodified proteins, the cyclodextrin-modified proteins (lysozyme and RNase A) exhibit significant reduction in aggregation, surface adsorption and increase in thermal stability. These results reveal a new chemistry for preventing protein aggregation and surface adsorption that is likely of different mechanisms than that by modifying proteins with poly(ethylene glycol).

Original language	English (US)
Pages (from-to)	13091-13096
Number of pages	6
Journal	Langmuir
Volume	27
Issue number	21
DOIs	https://doi.org/10.1021/la203271u
State	Published - Nov 1 2011

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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10.1021/la203271u

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abstract = "This work describes a general approach for preventing protein aggregation and surface adsorption by modifying proteins with β-cyclodextrins (βCD) via an efficient water-driven ligation. As compared to native unmodified proteins, the cyclodextrin-modified proteins (lysozyme and RNase A) exhibit significant reduction in aggregation, surface adsorption and increase in thermal stability. These results reveal a new chemistry for preventing protein aggregation and surface adsorption that is likely of different mechanisms than that by modifying proteins with poly(ethylene glycol).",

author = "Deepali Prashar and Dawei Cui and Debjyoti Bandyopadhyay and Luk, {Yan Yeung}",

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AU - Cui, Dawei

AU - Bandyopadhyay, Debjyoti

AU - Luk, Yan Yeung

PY - 2011/11/1

Y1 - 2011/11/1

N2 - This work describes a general approach for preventing protein aggregation and surface adsorption by modifying proteins with β-cyclodextrins (βCD) via an efficient water-driven ligation. As compared to native unmodified proteins, the cyclodextrin-modified proteins (lysozyme and RNase A) exhibit significant reduction in aggregation, surface adsorption and increase in thermal stability. These results reveal a new chemistry for preventing protein aggregation and surface adsorption that is likely of different mechanisms than that by modifying proteins with poly(ethylene glycol).

AB - This work describes a general approach for preventing protein aggregation and surface adsorption by modifying proteins with β-cyclodextrins (βCD) via an efficient water-driven ligation. As compared to native unmodified proteins, the cyclodextrin-modified proteins (lysozyme and RNase A) exhibit significant reduction in aggregation, surface adsorption and increase in thermal stability. These results reveal a new chemistry for preventing protein aggregation and surface adsorption that is likely of different mechanisms than that by modifying proteins with poly(ethylene glycol).

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Modification of proteins with cyclodextrins prevents aggregation and surface adsorption and increases thermal stability

Abstract

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