Modification of carboplatin by Jurkat cells

Anthony J. Di Pasqua, Jerry Goodisman, Deborah J. Kerwood, Bonnie B. Toms, James C. Dabrowiak

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Using [1H,15N] heteronuclear single quantum coherance (HSQC) NMR and 15N-labeled carboplatin, 1, we show that Jurkat cells affect the rate of disappearance of the HSQC NMR peak in culture medium for this Pt2+ anticancer drug. The decay or disappearance rate constant for 1 in culture medium containing cells is k1 = kc [CO32 -] + km + ku N, where kc is the rate constant for reaction of 1 with carbonate in the medium, km is the rate constant for reaction of 1 with all other components of the medium, and ku is the rate constant for reaction of 1 with cells having a number density N in the medium. Since Jurkat cells only take up a small amount of the platinum present in the medium (<1%), the observed disappearance of the HSQC NMR peak for 1 cannot be due to uptake of carboplatin by the cells.

Original languageEnglish (US)
Pages (from-to)1438-1441
Number of pages4
JournalJournal of Inorganic Biochemistry
Volume101
Issue number10
DOIs
StatePublished - Oct 2007

Keywords

  • Cells
  • NMR
  • Pt complex

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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    Di Pasqua, A. J., Goodisman, J., Kerwood, D. J., Toms, B. B., & Dabrowiak, J. C. (2007). Modification of carboplatin by Jurkat cells. Journal of Inorganic Biochemistry, 101(10), 1438-1441. https://doi.org/10.1016/j.jinorgbio.2007.06.018