Minimalist IR and fluorescence probes of protein function

Pallavi M. Gosavi, Ivan Korendovych

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

Spectroscopic studies of small proteins and peptides, especially those requiring fine spatial and/or temporal resolution, demand synthetic probes that confer the minimal possible steric and functional change on the native properties. Here we review the recent progress in development of minimally disruptive probes for fluorescence and infrared spectroscopies, as well as the methods to efficiently incorporate them into proteins. Advances in spectroscopy on the one hand result in high specialization of synthetic probes for a particular purpose, but on the other hand allow for the same probes be used for different techniques to gather complementary biochemical information.

Original languageEnglish (US)
Pages (from-to)103-109
Number of pages7
JournalCurrent Opinion in Chemical Biology
Volume34
DOIs
StatePublished - Oct 1 2016

Fingerprint

Fluorescence
Fluorescence Spectrometry
Spectrum Analysis
Proteins
Peptides
Fluorescence spectroscopy
Infrared spectroscopy
Spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Minimalist IR and fluorescence probes of protein function. / Gosavi, Pallavi M.; Korendovych, Ivan.

In: Current Opinion in Chemical Biology, Vol. 34, 01.10.2016, p. 103-109.

Research output: Contribution to journalReview article

@article{1b123d3ed7824e489a469d25e4a2a906,
title = "Minimalist IR and fluorescence probes of protein function",
abstract = "Spectroscopic studies of small proteins and peptides, especially those requiring fine spatial and/or temporal resolution, demand synthetic probes that confer the minimal possible steric and functional change on the native properties. Here we review the recent progress in development of minimally disruptive probes for fluorescence and infrared spectroscopies, as well as the methods to efficiently incorporate them into proteins. Advances in spectroscopy on the one hand result in high specialization of synthetic probes for a particular purpose, but on the other hand allow for the same probes be used for different techniques to gather complementary biochemical information.",
author = "Gosavi, {Pallavi M.} and Ivan Korendovych",
year = "2016",
month = "10",
day = "1",
doi = "10.1016/j.cbpa.2016.08.010",
language = "English (US)",
volume = "34",
pages = "103--109",
journal = "Current Opinion in Chemical Biology",
issn = "1367-5931",
publisher = "Elsevier",

}

TY - JOUR

T1 - Minimalist IR and fluorescence probes of protein function

AU - Gosavi, Pallavi M.

AU - Korendovych, Ivan

PY - 2016/10/1

Y1 - 2016/10/1

N2 - Spectroscopic studies of small proteins and peptides, especially those requiring fine spatial and/or temporal resolution, demand synthetic probes that confer the minimal possible steric and functional change on the native properties. Here we review the recent progress in development of minimally disruptive probes for fluorescence and infrared spectroscopies, as well as the methods to efficiently incorporate them into proteins. Advances in spectroscopy on the one hand result in high specialization of synthetic probes for a particular purpose, but on the other hand allow for the same probes be used for different techniques to gather complementary biochemical information.

AB - Spectroscopic studies of small proteins and peptides, especially those requiring fine spatial and/or temporal resolution, demand synthetic probes that confer the minimal possible steric and functional change on the native properties. Here we review the recent progress in development of minimally disruptive probes for fluorescence and infrared spectroscopies, as well as the methods to efficiently incorporate them into proteins. Advances in spectroscopy on the one hand result in high specialization of synthetic probes for a particular purpose, but on the other hand allow for the same probes be used for different techniques to gather complementary biochemical information.

UR - http://www.scopus.com/inward/record.url?scp=84985007040&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84985007040&partnerID=8YFLogxK

U2 - 10.1016/j.cbpa.2016.08.010

DO - 10.1016/j.cbpa.2016.08.010

M3 - Review article

C2 - 27599185

AN - SCOPUS:84985007040

VL - 34

SP - 103

EP - 109

JO - Current Opinion in Chemical Biology

JF - Current Opinion in Chemical Biology

SN - 1367-5931

ER -