TY - JOUR
T1 - Microfluidic characterization of specific membrane capacitance and cytoplasm conductivity of single cells
AU - Zheng, Yi
AU - Shojaei-Baghini, Ehsan
AU - Wang, Chen
AU - Sun, Yu
N1 - Funding Information:
Financial support from the Natural Sciences and Engineering Research Council of Canada (NSERC) through a Strategic Grant, from the University of Toronto through a Connaught Innovation Award, and from the Canada Research Chairs Program is acknowledged.
PY - 2013/4/5
Y1 - 2013/4/5
N2 - This paper presents a technique for single-cell electrical property (specific membrane capacitance and cytoplasm conductivity) characterization at a speed of 5-10cells/s (vs. minutes/cell using existing techniques such as patch clamping and electrorotation). When a cell flows through a microfluidic constriction channel which is marginally smaller than the diameter of tested cells, electrical impedance at multiple frequencies is measured. Electrical and geometrical models are developed to interpret the impedance data and to determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3249 AML-2 cells and 3398 HL-60 cells reveal different specific membrane capacitance and cytoplasm conductivity values between AML-2 (12.0±1.44mF/m2, 0.62±0.10S/m) and HL-60 (14.5±1.75mF/m2, 0.76±0.12S/m) cells. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results since these parameters contain information additional to cell size.
AB - This paper presents a technique for single-cell electrical property (specific membrane capacitance and cytoplasm conductivity) characterization at a speed of 5-10cells/s (vs. minutes/cell using existing techniques such as patch clamping and electrorotation). When a cell flows through a microfluidic constriction channel which is marginally smaller than the diameter of tested cells, electrical impedance at multiple frequencies is measured. Electrical and geometrical models are developed to interpret the impedance data and to determine the specific membrane capacitance and cytoplasm conductivity of individual cells. Results from testing 3249 AML-2 cells and 3398 HL-60 cells reveal different specific membrane capacitance and cytoplasm conductivity values between AML-2 (12.0±1.44mF/m2, 0.62±0.10S/m) and HL-60 (14.5±1.75mF/m2, 0.76±0.12S/m) cells. The results also demonstrate that the quantification of specific membrane capacitance and cytoplasm conductivity can enhance cell classification results since these parameters contain information additional to cell size.
KW - Cell classification
KW - Electrical characterization
KW - High-throughput
KW - Impedance spectroscopy
KW - Microfluidics
KW - Single cells
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U2 - 10.1016/j.bios.2012.10.081
DO - 10.1016/j.bios.2012.10.081
M3 - Article
C2 - 23246657
AN - SCOPUS:84871667030
SN - 0956-5663
VL - 42
SP - 496
EP - 502
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 1
ER -