TY - JOUR
T1 - Microarray analysis of gene expression in the blue catfish liver reveals early activation of the MHC class I pathway after infection with Edwardsiella ictaluri
AU - Peatman, Eric
AU - Terhune, Jeffery
AU - Baoprasertkul, Puttharat
AU - Xu, Peng
AU - Nandi, Samiran
AU - Wang, Shaolin
AU - Somridhivej, Benjaporn
AU - Kucuktas, Huseyin
AU - Li, Ping
AU - Dunham, Rex
AU - Liu, Zhanjiang
N1 - Funding Information:
This project was supported by a grant from USDA NRI Animal Genome Tools and Resources Program (award # 2006-35616-16685), and in part by a seed grant from the AAES Foundation. We are grateful for an equipment grant from the National Research Initiative Competitive Grant no. 2005-35206-15274 from the USDA Cooperative State Research, Education, and Extension Service. We thank Renee Beam, Esau Arana, and Randell Goodman for their excellence in the production and maintenance of fish used in this study and their assistance during challenge experiments. We are grateful to Karl Hayden of the Fish Disease Diagnostic Laboratory for his assistance in the diagnosis of the ESC disease and the identification of the bacterial pathogen.
PY - 2008/1
Y1 - 2008/1
N2 - The acute nature of disease outbreaks in aquaculture settings has served to emphasize the importance of the innate immune response of fish for survival and led to the recent identification and characterization of many of its components. Catfish, the predominant aquaculture species in the United States, is an important model for the study of the teleost immune system. However, transcriptomic-level studies of disease-related gene expression in catfish have only recently been initiated, and understanding of immune responses to pathogen infections is limited. Here, we have developed and utilized a 28K in situ oligonucleotide microarray composed of blue catfish (Ictalurus furcatus) and channel catfish (Ictalurus punctatus) transcripts. While channel catfish accounts for the majority of commercial production, the closely related blue catfish possesses several economically important phenotypic traits. Microarray analysis of gene expression changes in blue catfish liver after infection with Gram-negative bacterium Edwardsiella ictaluri indicated the strong upregulation of several pathways involved in the inflammatory immune response and potentially in innate disease resistance. A multifaceted response to infection could be observed, encompassing the complement cascade, iron regulation, inflammatory cell signaling, and antigen processing and presentation. The induction of several components of the MHC class I-related pathway following infection with an intracellular bacterium is reported here for the first time in fish. A comparison with previously published expression profiles in the channel catfish liver was also made and the microarray results extended by use of quantitative RT-PCR. Our results add to the understanding of the teleost immune responses and provide a solid foundation for future functional characterization, genetic mapping, and QTL analysis of immunity-related genes from catfish.
AB - The acute nature of disease outbreaks in aquaculture settings has served to emphasize the importance of the innate immune response of fish for survival and led to the recent identification and characterization of many of its components. Catfish, the predominant aquaculture species in the United States, is an important model for the study of the teleost immune system. However, transcriptomic-level studies of disease-related gene expression in catfish have only recently been initiated, and understanding of immune responses to pathogen infections is limited. Here, we have developed and utilized a 28K in situ oligonucleotide microarray composed of blue catfish (Ictalurus furcatus) and channel catfish (Ictalurus punctatus) transcripts. While channel catfish accounts for the majority of commercial production, the closely related blue catfish possesses several economically important phenotypic traits. Microarray analysis of gene expression changes in blue catfish liver after infection with Gram-negative bacterium Edwardsiella ictaluri indicated the strong upregulation of several pathways involved in the inflammatory immune response and potentially in innate disease resistance. A multifaceted response to infection could be observed, encompassing the complement cascade, iron regulation, inflammatory cell signaling, and antigen processing and presentation. The induction of several components of the MHC class I-related pathway following infection with an intracellular bacterium is reported here for the first time in fish. A comparison with previously published expression profiles in the channel catfish liver was also made and the microarray results extended by use of quantitative RT-PCR. Our results add to the understanding of the teleost immune responses and provide a solid foundation for future functional characterization, genetic mapping, and QTL analysis of immunity-related genes from catfish.
KW - Catfish
KW - Disease
KW - Genome
KW - Immunity
KW - Infection
KW - Microarray
KW - Transcriptome
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UR - http://www.scopus.com/inward/citedby.url?scp=34548356906&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2007.05.012
DO - 10.1016/j.molimm.2007.05.012
M3 - Article
C2 - 17599411
AN - SCOPUS:34548356906
SN - 0161-5890
VL - 45
SP - 553
EP - 566
JO - Molecular Immunology
JF - Molecular Immunology
IS - 2
ER -