Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer

Angela S. Lindner, Adriana Pacheco, Henry C. Aldrich, Andria Costello Staniec, Ilker Uz, David J. Hodson

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Strain CSC1T, a Gram-negative, aerobic, methane-oxidizing bacterium, was isolated from an uncontaminated aquifer nearly 20 years ago. Based on 16S rRNA gene sequence similarity, this strain was identified as a member of the Alphaproteobacteria, most closely related to an uncultured member of the Methylocystaceae as well as two cultured organisms, Methylocystis sp. L32 and Methylocystis sp. SC2. This strain differed from extant species in cell shape, size, expression of soluble methane monooxygenase and its unique spiny surface layers, composed of polysaccharide. DNA-DNA hybridization results showed only 3.8 % relatedness with Methylocystis echinoides NCIMB 13100 and 41.1 % relatedness with Methylocystis rosea SV97T. Based on these genotypic and physiological differences, this isolate is proposed as a member of a novel species of the genus Methylocystis, Methylocystis hirsura sp. nov. (type strain CSC1T =ATCC BAA-1344T =DSM 18500T).

Original languageEnglish (US)
Pages (from-to)1891-1900
Number of pages10
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume57
Issue number8
DOIs
StatePublished - Aug 2007

Fingerprint

Methylocystaceae
Methylocystis
methanotrophs
Groundwater
relatedness
aquifers
groundwater
methane
aquifer
DNA
new species
polysaccharide
surface layer
Methylocystis echinoides
methane monooxygenase
bacterium
gene
Methylococcaceae
alpha-Proteobacteria
nucleic acid hybridization

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer. / Lindner, Angela S.; Pacheco, Adriana; Aldrich, Henry C.; Staniec, Andria Costello; Uz, Ilker; Hodson, David J.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 57, No. 8, 08.2007, p. 1891-1900.

Research output: Contribution to journalArticle

Lindner, Angela S. ; Pacheco, Adriana ; Aldrich, Henry C. ; Staniec, Andria Costello ; Uz, Ilker ; Hodson, David J. / Methylocystis hirsuta sp. nov., a novel methanotroph isolated from a groundwater aquifer. In: International Journal of Systematic and Evolutionary Microbiology. 2007 ; Vol. 57, No. 8. pp. 1891-1900.
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