Methods to analyze novel liaisons between endosomes and centrosomes

Hui Fang Hung, Heidi Hehnly, Stephen Doxsey

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations

Abstract

For some time, it has been known that recycling endosomes (REs) are organized in a nebulous "pericentrosomal" region in interphase cells. However, the collective use of previously developed methods, including centrosome isolation, live cell imaging, and electron microscopy, suggested that there is much more going on between the centrosome and the RE than previously imagined. By exploiting these approaches, we uncovered novel roles of the centrosome in RE function and, conversely, novel roles for REs in centrosome function. We first found that REs dynamically localized to the centrosome. throughout the cell cycle. More specifically, we found that REs interacted with appendages of the older centriole in interphase cells to control endosome recycling, and this interaction was governed by RE-machinery including the small GTPase Rab11. We next determined that REs carry centrosome proteins to spindle poles as part of the "centrosome maturation" process. Here we discuss the methods used and materials needed to complete these types of studies.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
Subtitle of host publicationSorting and Recycling Endosomes, 2015
EditorsWei Guo
PublisherAcademic Press Inc.
Pages47-58
Number of pages12
ISBN (Print)9780128028292
DOIs
StatePublished - 2015

Publication series

NameMethods in Cell Biology
Volume130
ISSN (Print)0091-679X

Keywords

  • Appendages
  • Cenexin
  • Centriolin
  • Centrosome
  • Centrosome isolation
  • Evi5
  • Fip3
  • Mitosis
  • Mother centriole
  • Rab11
  • Rab8
  • Recycling endosome
  • Spindle pole

ASJC Scopus subject areas

  • Cell Biology

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