Metal-citrate complex transport in Kineococcus radiotolerans

Brian P. Huta, Nigel H. Miller, Eleanor L. Robertson, Robert Patrick Doyle

Research output: Contribution to journalArticle

Abstract

The growth of an organism is highly dependent on the acquisition of carbon and metals, and availability of these nutrients in the environment affects its survival. Organisms can obtain both nutrients simultaneously through proteins of the CitMHS superfamily. Bioinformatic studies suggested a CitMHS gene (Accession number ABS03965.1) in Kineococcus radiotolerans. Radio flux assays following 14-C radiolabelled citrate, either free or complexed to a variety of metal ions, in K. radiotolerans demonstrated internalization of the citrate when bound to select metal ions only, primarily in the form of calcium-citrate. A pH response was also observed, consistent with a permease (ATP independent) mechanism as noted for other CitMHS family members, with greater uptake at pH 7 compared to pH 10. These results confirm the ability of K. radiotolerans to transport complexed citrate.

Original languageEnglish (US)
JournalJournal of Basic Microbiology
DOIs
StateAccepted/In press - Jan 1 2017

Fingerprint

Coordination Complexes
Citric Acid
Metals
Calcium Citrate
Ions
Food
Aptitude
Membrane Transport Proteins
Computational Biology
Radio
Carbon
Adenosine Triphosphate
Growth
Genes
Proteins

Keywords

  • CitMHS
  • Metal-citrate complex
  • Symport

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Metal-citrate complex transport in Kineococcus radiotolerans. / Huta, Brian P.; Miller, Nigel H.; Robertson, Eleanor L.; Doyle, Robert Patrick.

In: Journal of Basic Microbiology, 01.01.2017.

Research output: Contribution to journalArticle

@article{7b2f2ca6c8444654b0bfa14541c65b5e,
title = "Metal-citrate complex transport in Kineococcus radiotolerans",
abstract = "The growth of an organism is highly dependent on the acquisition of carbon and metals, and availability of these nutrients in the environment affects its survival. Organisms can obtain both nutrients simultaneously through proteins of the CitMHS superfamily. Bioinformatic studies suggested a CitMHS gene (Accession number ABS03965.1) in Kineococcus radiotolerans. Radio flux assays following 14-C radiolabelled citrate, either free or complexed to a variety of metal ions, in K. radiotolerans demonstrated internalization of the citrate when bound to select metal ions only, primarily in the form of calcium-citrate. A pH response was also observed, consistent with a permease (ATP independent) mechanism as noted for other CitMHS family members, with greater uptake at pH 7 compared to pH 10. These results confirm the ability of K. radiotolerans to transport complexed citrate.",
keywords = "CitMHS, Metal-citrate complex, Symport",
author = "Huta, {Brian P.} and Miller, {Nigel H.} and Robertson, {Eleanor L.} and Doyle, {Robert Patrick}",
year = "2017",
month = "1",
day = "1",
doi = "10.1002/jobm.201700427",
language = "English (US)",
journal = "Journal of Basic Microbiology",
issn = "0233-111X",
publisher = "Wiley-VCH Verlag",

}

TY - JOUR

T1 - Metal-citrate complex transport in Kineococcus radiotolerans

AU - Huta, Brian P.

AU - Miller, Nigel H.

AU - Robertson, Eleanor L.

AU - Doyle, Robert Patrick

PY - 2017/1/1

Y1 - 2017/1/1

N2 - The growth of an organism is highly dependent on the acquisition of carbon and metals, and availability of these nutrients in the environment affects its survival. Organisms can obtain both nutrients simultaneously through proteins of the CitMHS superfamily. Bioinformatic studies suggested a CitMHS gene (Accession number ABS03965.1) in Kineococcus radiotolerans. Radio flux assays following 14-C radiolabelled citrate, either free or complexed to a variety of metal ions, in K. radiotolerans demonstrated internalization of the citrate when bound to select metal ions only, primarily in the form of calcium-citrate. A pH response was also observed, consistent with a permease (ATP independent) mechanism as noted for other CitMHS family members, with greater uptake at pH 7 compared to pH 10. These results confirm the ability of K. radiotolerans to transport complexed citrate.

AB - The growth of an organism is highly dependent on the acquisition of carbon and metals, and availability of these nutrients in the environment affects its survival. Organisms can obtain both nutrients simultaneously through proteins of the CitMHS superfamily. Bioinformatic studies suggested a CitMHS gene (Accession number ABS03965.1) in Kineococcus radiotolerans. Radio flux assays following 14-C radiolabelled citrate, either free or complexed to a variety of metal ions, in K. radiotolerans demonstrated internalization of the citrate when bound to select metal ions only, primarily in the form of calcium-citrate. A pH response was also observed, consistent with a permease (ATP independent) mechanism as noted for other CitMHS family members, with greater uptake at pH 7 compared to pH 10. These results confirm the ability of K. radiotolerans to transport complexed citrate.

KW - CitMHS

KW - Metal-citrate complex

KW - Symport

UR - http://www.scopus.com/inward/record.url?scp=85038389433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85038389433&partnerID=8YFLogxK

U2 - 10.1002/jobm.201700427

DO - 10.1002/jobm.201700427

M3 - Article

C2 - 29226973

AN - SCOPUS:85038389433

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 0233-111X

ER -