Membrane transport in synchronized Ehrlich ascites tumor cells

uptake of amino acids by the A and L system during the cell cycle

J. T. Tupper, B. Mills, F. Zorgniotti

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Using the double thymidine block technique, Ehrlich ascites tumor cells (ELD) carried in continuous spinner culture were synchronized. Simultaneous monitoring of 3H thymidine incorporation, cell number and mitotic index yielded a cell cycle time of approximately 13.5 hr. This is composed of an S period of 3-4 hr, G2 of 6-8 hr and M of 1-2 hr. No appreciable G1 is present. Ehrlich cells synchronized in this manner were used to investigate the characteristics of two neutral amino acid transport systems during progression through the cell cycle. Unidirectional influx via the Na dependent system A was studied using C14 alpha aminoisobutyrate (AIB) as substrate. The Na independent system L was monitored using 3H leucine and 14C cycloleucine as substrates. Transport by the A system was minimal in M and early S. It underwent a three fold increase during late S and early G2. In mid G2 the transport via this system rapidly dropped and remained low again through M and early S. The intracellular/extracellular ratios of AIB indicate that the system is actively transporting AIB throughout the cell cycle. The minimum ratios of approximately 3 were achieved during early M and the maximum ratios of approximately 9 were achieved in late S, early G2. The uptake of leucine and cycloleucine by the L system was quite different during the cell cycle. Maximal unidirectional influx by this system occurred during early and mid S period. Upon progression into G2 the transport rate dropped and remained reduced throughout M. Intracellular/ extracellular ratios of leucine or cycloleucine were near unity at the peak of the transport activity (early S) and dropped to values of 0.5 to 0.6 throughout the remainder of the cycle. This result indicates that inward transport by the L system is, for the most part, non active in growing cells.

Original languageEnglish (US)
Pages (from-to)77-87
Number of pages11
JournalJournal of Cellular Physiology
Volume88
Issue number1
StatePublished - 1976

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Ehrlich Tumor Carcinoma
Cycloleucine
Tumors
Cell Cycle
Cells
Leucine
Membranes
Amino Acids
S Phase
Thymidine
Neutral Amino Acid Transport Systems
Mitotic Index
Substrates
Cell culture
Cell Count
Monitoring

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Membrane transport in synchronized Ehrlich ascites tumor cells : uptake of amino acids by the A and L system during the cell cycle. / Tupper, J. T.; Mills, B.; Zorgniotti, F.

In: Journal of Cellular Physiology, Vol. 88, No. 1, 1976, p. 77-87.

Research output: Contribution to journalArticle

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