TY - GEN
T1 - Mechanically guided emergent patterning of neuroectoderm tissue using human pluripotent stem cells
AU - Xue, Xufeng
AU - Sun, Yubing
AU - Resto-Irizarry, Agnes
AU - Yong, Koh Meng Aw
AU - Zheng, Yi
AU - Weng, Shinuo
AU - Shao, Yue
AU - Fu, Jianping
N1 - Funding Information:
This work is supported in part by the National Science Foundation (CMMI 1129611 and CBET 1149401), the American Heart Association (12SDG12180025), and the Department of Mechanical Engineering at the University of Michigan, Ann Arbor. The Lurie Nanofabrication Facility at tthe University of Michigan, a member of the National Nanotechnology Infrastructure Network (NNIN) funded by the National Science Foundation, is acknowledged for support in microfabrication.
Publisher Copyright:
© 17CBMS-0001.
PY - 2020
Y1 - 2020
N2 - Classic embryological studies have successfully applied genetics and cell biology principles to understand embryology. However, it remains unresolved how tissue-scale morphogenetic events, as an integral part for shaping development, are involved in embryonic patterning. Herein, we generated for the first time in vitro neuroectoderm tissues with spatially patterned neural plate (NP) and neural plate border (NPB) cells by differentiating human pluripotent stem cells (hPSCs) on geometrically confined substrates. We depicted the critical roles of mechanical cues, including cytoskeletal contractility and cell shape, and their cross-talk with BMP signaling, in guiding the emergent patterning of neuroectoderm tissue from hPSCs.
AB - Classic embryological studies have successfully applied genetics and cell biology principles to understand embryology. However, it remains unresolved how tissue-scale morphogenetic events, as an integral part for shaping development, are involved in embryonic patterning. Herein, we generated for the first time in vitro neuroectoderm tissues with spatially patterned neural plate (NP) and neural plate border (NPB) cells by differentiating human pluripotent stem cells (hPSCs) on geometrically confined substrates. We depicted the critical roles of mechanical cues, including cytoskeletal contractility and cell shape, and their cross-talk with BMP signaling, in guiding the emergent patterning of neuroectoderm tissue from hPSCs.
KW - BMP-Smad signaling
KW - Human pluripotent stem cells
KW - Mechanobiology
KW - Neuroectoderm
UR - http://www.scopus.com/inward/record.url?scp=85079662796&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079662796&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079662796
T3 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
SP - 145
EP - 146
BT - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PB - Chemical and Biological Microsystems Society
T2 - 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Y2 - 22 October 2017 through 26 October 2017
ER -