Mechanically guided emergent patterning of neuroectoderm tissue using human pluripotent stem cells

Xufeng Xue, Yubing Sun, Agnes Resto-Irizarry, Koh Meng Aw Yong, Yi Zheng, Shinuo Weng, Yue Shao, Jianping Fu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Classic embryological studies have successfully applied genetics and cell biology principles to understand embryology. However, it remains unresolved how tissue-scale morphogenetic events, as an integral part for shaping development, are involved in embryonic patterning. Herein, we generated for the first time in vitro neuroectoderm tissues with spatially patterned neural plate (NP) and neural plate border (NPB) cells by differentiating human pluripotent stem cells (hPSCs) on geometrically confined substrates. We depicted the critical roles of mechanical cues, including cytoskeletal contractility and cell shape, and their cross-talk with BMP signaling, in guiding the emergent patterning of neuroectoderm tissue from hPSCs.

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages145-146
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - 2020
Externally publishedYes
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
Country/TerritoryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • BMP-Smad signaling
  • Human pluripotent stem cells
  • Mechanobiology
  • Neuroectoderm

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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