Measuring transport of motor cargos.

Leslie Conway, Jennifer L. Ross

Research output: Contribution to journalReview article

Abstract

In this chapter, we describe experimental techniques used in vitro to illuminate how small teams of motors can work to translocate cargos. We will focus on experiments utilizing in vitro reconstitution, artificial or ex vivo purified cargos, and fluorescence imaging. A number of studies have been able to recapitulate the activities of cargo transport driven by small teams of motors elucidating how multiple motors can work together to transport cargos within the cell. Here, we describe some of the methods employed and highlight important experimental details needed to perform these experiments.

Original languageEnglish (US)
Pages (from-to)235-252
Number of pages18
JournalEXS
Volume105
DOIs
StatePublished - 2014

ASJC Scopus subject areas

  • Medicine(all)

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