TY - JOUR
T1 - Measuring transport of motor cargos.
AU - Conway, Leslie
AU - Ross, Jennifer L.
PY - 2014
Y1 - 2014
N2 - In this chapter, we describe experimental techniques used in vitro to illuminate how small teams of motors can work to translocate cargos. We will focus on experiments utilizing in vitro reconstitution, artificial or ex vivo purified cargos, and fluorescence imaging. A number of studies have been able to recapitulate the activities of cargo transport driven by small teams of motors elucidating how multiple motors can work together to transport cargos within the cell. Here, we describe some of the methods employed and highlight important experimental details needed to perform these experiments.
AB - In this chapter, we describe experimental techniques used in vitro to illuminate how small teams of motors can work to translocate cargos. We will focus on experiments utilizing in vitro reconstitution, artificial or ex vivo purified cargos, and fluorescence imaging. A number of studies have been able to recapitulate the activities of cargo transport driven by small teams of motors elucidating how multiple motors can work together to transport cargos within the cell. Here, we describe some of the methods employed and highlight important experimental details needed to perform these experiments.
UR - http://www.scopus.com/inward/record.url?scp=84907753157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907753157&partnerID=8YFLogxK
U2 - 10.1007/978-3-0348-0856-9_11
DO - 10.1007/978-3-0348-0856-9_11
M3 - Review article
C2 - 25095998
AN - SCOPUS:84907753157
VL - 105
SP - 235
EP - 252
JO - Experientia. Supplementum
JF - Experientia. Supplementum
SN - 1023-294X
ER -