MBD2 and MeCP2 regulate distinct transitions in the stage-specific differentiation of olfactory receptor neurons

Jessica L. MacDonald, Adrian Verster, Anthony Berndt, A. Jane Roskams

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

DNA methylation-dependent gene silencing is initiated by DNA methyltransferases (DNMTs) and mediated by methyl-binding domain proteins (MBDs), which recruit histone deacetylases (HDACs) to silence DNA, a process that is essential for normal development. Here, we show that the MBD proteins MBD2 and MeCP2 regulate distinct transitional stages of olfactory receptor neuron (ORN) differentiation in vivo. Mbd2 null progenitors display enhanced proliferation, recapitulated by HDAC inhibition, and Mbd2 null ORNs have a decreased lifespan. Mecp2 null ORNs, on the other hand, temporarily stall at the stage of terminal differentiation, retaining expression of the immature neuronal protein GAP43 after initiating expression of mature neuronal genes. The Gap43 promoter is highly methylated in the mature, but not embryonic olfactory epithelium (OE), suggesting that Gap43 may be regulated by DNA methylation during ORN differentiation. Thus, MBD2 and MeCP2 may mediate distinct, sequential transitions of ORN differentiation-an epigenetic mechanism that may be relevant to developmental regulation throughout the nervous system.

Original languageEnglish (US)
Pages (from-to)55-67
Number of pages13
JournalMolecular and Cellular Neuroscience
Volume44
Issue number1
DOIs
StatePublished - May 1 2010
Externally publishedYes

Keywords

  • Epigenetics
  • Histone deacetylase
  • Neural development
  • Neurogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Fingerprint Dive into the research topics of 'MBD2 and MeCP2 regulate distinct transitions in the stage-specific differentiation of olfactory receptor neurons'. Together they form a unique fingerprint.

  • Cite this