Major histocompatibility complex class IIB allele polymorphism and its association with resistance/susceptibility to Vibrio anguillarum in Japanese flounder (Paralichthys olivaceus)

Y. X. Zhang, S. L. Chen, Y. G. Liu, Z. X. Sha, Z. J. Liu

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

The full length of major histocompatibility complex (MHC) class IIB cDNA was cloned from a Chinese population of Paralichthys olivaceus by homology cloning and rapid amplification of cDNA ends-polymerase chain reaction (RACE-PCR). The MHC IIB genomic sequence is 1,864 bp long and consists of 34-bp 5′UTR, 741-bp open reading frame, 407-bp 3′UTR, 96-bp intron1, 392-bp intron2, 85-bp intron3, and 109-bp intron4. Phylogenetic analysis showed that the putative MHC class IIB amino acid of the Chinese P. olivaceus shared 28.3% to 85.4% identity with that of the reported MHC class IIB in other species. A significant association between MHC IIB polymorphism and disease resistance/susceptibility was found in Chinese P. olivaceus. Thirteen different MHC IIB alleles were identified among 411 clones from 84 individuals. Among the 280 (268) nucleotides, 32 (11.4%) nucleotide positions were variable. Most alleles such as alleles a, b, c, d, e, f, j, k, i, m were commonly found in both resistant and susceptible stock. Via χ 2 test, allele d was significantly more prevalent in individuals from susceptible stock than from resistant stock, and their percentages were 23.80% and 7.14%, respectively. In addition, allele g occurred in 9 and allele h in 4 of 42 resistant individuals that were not present in the susceptible stock; their percentages were 21.4% and 9.52%, respectively. Although allele l was found only in 8 individuals from the susceptible stock, its percentage is 19.05%.

Original languageEnglish (US)
Pages (from-to)600-610
Number of pages11
JournalMarine Biotechnology
Volume8
Issue number6
DOIs
StatePublished - Dec 1 2006
Externally publishedYes

Keywords

  • Disease resistance
  • Disease susceptibility
  • Japanese flounder
  • Major histocompatibility complex class IIB (MHC IIB)
  • Paralichthys olivaceus
  • Polymorphism
  • cDNA

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

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