Maize Spm transposable element has an enhancer-insensitive promoter

Ramesh Raina, Douglas Cook, Nina Fedoroff

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have used a transient assay system to investigate the promoter region of the maize Suppressor-mutator (Spm) transposable element. All of the sequence required for constitutive promoter activity is confined to the 0.2-kb sequence upstream from the transcription start site of the element at nt 209 and designated the upstream control region. The element's promoter is weak, lacks a conventional TATA box, and depends on the presence of multiple, short repetitive sequence elements. The Spm promoter is quite insensitive to the enhancer sequence of the cauliflower mosaic virus 35S promoter. Enhancer sensitivity can be restored by providing a -30 TATA sequence and removing the G+C-rich sequence encoding the untranslated leader of the element, designated the downstream control region. Although the downstream control region is without effect on Spm promoter activity, it completely inhibits the 35S core promoter and markedly inhibits activity of the complete 35S promoter. The properties of the Spm element's promoter buffer it from both mutational and position-dependent changes in activity. We suggest that the inherent characteristics of the promoter are part of the genetic mechanism that controls the element's transposition frequency, ensuring it remains low and insertion-site independent.

Original languageEnglish (US)
Pages (from-to)6355-6359
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number13
StatePublished - Jul 1 1993
Externally publishedYes

Fingerprint

DNA Transposable Elements
Genetic Promoter Regions
Zea mays
Caulimovirus
TATA Box
Nucleic Acid Repetitive Sequences
Transcription Initiation Site
Buffers

Keywords

  • Luciferase gene
  • Particle bombardment
  • Plant promoters
  • Transcription regulation
  • Transient assay

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Maize Spm transposable element has an enhancer-insensitive promoter. / Raina, Ramesh; Cook, Douglas; Fedoroff, Nina.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 13, 01.07.1993, p. 6355-6359.

Research output: Contribution to journalArticle

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N2 - We have used a transient assay system to investigate the promoter region of the maize Suppressor-mutator (Spm) transposable element. All of the sequence required for constitutive promoter activity is confined to the 0.2-kb sequence upstream from the transcription start site of the element at nt 209 and designated the upstream control region. The element's promoter is weak, lacks a conventional TATA box, and depends on the presence of multiple, short repetitive sequence elements. The Spm promoter is quite insensitive to the enhancer sequence of the cauliflower mosaic virus 35S promoter. Enhancer sensitivity can be restored by providing a -30 TATA sequence and removing the G+C-rich sequence encoding the untranslated leader of the element, designated the downstream control region. Although the downstream control region is without effect on Spm promoter activity, it completely inhibits the 35S core promoter and markedly inhibits activity of the complete 35S promoter. The properties of the Spm element's promoter buffer it from both mutational and position-dependent changes in activity. We suggest that the inherent characteristics of the promoter are part of the genetic mechanism that controls the element's transposition frequency, ensuring it remains low and insertion-site independent.

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