Lung cancer inhibitory effect of epigallocatechin-3-gallate is dependent on its presence in a complex mixture (polyphenon E)

Huijing Fu, Jun He, Fan Mei, Qi Zhang, Yukihiko Hara, Seto Ryota, Ronald A. Lubet, Ruth Chen, Da Ren Chen, Ming You

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Green tea has been shown to exhibit cancer-preventive activities in preclinical studies. However, (-)-epigallocatechin-3-gallate (EGCG) alone was shown to be ineffective in preventing lung tumorigenesis in mice by aerosol administration. In this study, Polyphenon E and Polyphenon E without EGCG were administered by aerosol delivery to A/J mice 2 weeks after carcinogen treatment and continuing daily throughout the remainder of the study (20 weeks). An improved aerosol delivery system with a custom-built atomizer, an efficient solvent remove system, and a nose-only exposure chamber was used to provide aerosols with stable size distribution. There were no significant differences in the size distributions of Polyphenon E and Polyphenon E without EGCG. With a relatively low dose level (4.19 mg/kg), Polyphenon E decreased tumor multiplicity by 53%, whereas Polyphenon E without EGCG at the same dose failed to inhibit lung carcinogenesis. These results indicate that aerosol administration can be an effective approach in chemoprevention study, and aerosolized Polyphenon E can significantly inhibit pulmonary adenoma formation and growth in A/J mice. Furthermore, in aerosolized form, EGCG, which is thought to be the most active component of Polyphenon E, has to be present with other tea catechins to show chemopreventive activity on lung tumorigenesis.

Original languageEnglish (US)
Pages (from-to)531-537
Number of pages7
JournalCancer Prevention Research
Volume2
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

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Complex Mixtures
Lung Neoplasms
Aerosols
Lung
Carcinogenesis
Tea
epigallocatechin gallate
polyphenon E
Catechin
Nebulizers and Vaporizers
Chemoprevention
Nose
Carcinogens
Adenoma
Neoplasms
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Lung cancer inhibitory effect of epigallocatechin-3-gallate is dependent on its presence in a complex mixture (polyphenon E). / Fu, Huijing; He, Jun; Mei, Fan; Zhang, Qi; Hara, Yukihiko; Ryota, Seto; Lubet, Ronald A.; Chen, Ruth; Chen, Da Ren; You, Ming.

In: Cancer Prevention Research, Vol. 2, No. 6, 06.2009, p. 531-537.

Research output: Contribution to journalArticle

Fu, Huijing ; He, Jun ; Mei, Fan ; Zhang, Qi ; Hara, Yukihiko ; Ryota, Seto ; Lubet, Ronald A. ; Chen, Ruth ; Chen, Da Ren ; You, Ming. / Lung cancer inhibitory effect of epigallocatechin-3-gallate is dependent on its presence in a complex mixture (polyphenon E). In: Cancer Prevention Research. 2009 ; Vol. 2, No. 6. pp. 531-537.
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