Low-copy microsatellite markers for Pinus taeda L.

Christine G. Elsik, Virginia T. Minihan, Sarah E. Hall, Ann M. Scarpa, Claire G. Williams

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Eighteen low-copy and genomic microsatellite markers were tested for Mendelian inheritance and then assayed in 41 Pinus taeda L. samples drawn from five regions in the southern United States. The PCR products had multiple alleles, high levels of polymorphism, and little non-specific priming. Fifteen of the 18 markers were informative for a P. taeda three-generation RFLP (restriction fragment length polymorphism) pedigree, and a P. taeda population survey revealed three to 28 alleles per locus. The highest allele numbers and polymorphic information content (PIC) values were associated with complex repeat sequences and (or) with sequences consisting of the longer strings of perfect repeats. The abundance of low- to rare-frequency alleles also accounted for high PIC values in both types of markers. Low-copy microsatellites are useful for the large, complex pine genome, especially in the absence of entire gene sequences in public databases and with the low levels of polymorphism in markers developed from expressed sequence tags (ESTs).

Original languageEnglish (US)
Pages (from-to)550-555
Number of pages6
JournalGenome
Volume43
Issue number3
StatePublished - Aug 21 2000
Externally publishedYes

Keywords

  • Conifers
  • Gymnosperms
  • Loblolly pine
  • Trinucleotide repeat motifs

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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  • Cite this

    Elsik, C. G., Minihan, V. T., Hall, S. E., Scarpa, A. M., & Williams, C. G. (2000). Low-copy microsatellite markers for Pinus taeda L. Genome, 43(3), 550-555.