@inproceedings{21fd0e3d19554c8192e7a365e8982ef6,
title = "Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy",
abstract = "Utilizing a custom-built, on-stage incubator-combined, two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging system, we observed new-sarcomere addition in rat neonatal cardiomyocytes during 10 hours of on-stage incubation. This addition occurred at one end of an existing myofibril, the sides of existing myofibrils, and at the interstice of several separated myofibrils; in the cases of the latter two, we observed mature myofibrils acting as templates. We found that during sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric length during maturation.",
keywords = "myofibrillogenesis, myosin filaments, on-stage incubator, sarcomere addition, second harmonic generation",
author = "Honghai Liu and Wan Qin and Yonghong Shao and Qiuying Liu and Zhen Ma and Borg, {Thomas K.} and Gao, {Bruce Z.}",
year = "2012",
doi = "10.1117/12.908381",
language = "English (US)",
isbn = "9780819488688",
series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",
booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X",
note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X ; Conference date: 21-01-2012 Through 23-01-2012",
}