Live cardiomyocyte imaging via hybrid TPEF-SHG microscopy

Honghai Liu, Wan Qin, Yonghong Shao, Qiuying Liu, Zhen Ma, Thomas K. Borg, Bruce Z. Gao

Research output: Chapter in Book/Entry/PoemConference contribution

5 Scopus citations


Utilizing a custom-built, on-stage incubator-combined, two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) imaging system, we observed new-sarcomere addition in rat neonatal cardiomyocytes during 10 hours of on-stage incubation. This addition occurred at one end of an existing myofibril, the sides of existing myofibrils, and at the interstice of several separated myofibrils; in the cases of the latter two, we observed mature myofibrils acting as templates. We found that during sarcomeric addition, myosin filaments are assembled onto the premyofibril laterally. This lateral addition, which proceeds stepwise along the axial direction, plays an important role in the accumulation of Z-bodies to form mature Z-disks and in the regulation of sarcomeric length during maturation.

Original languageEnglish (US)
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
StatePublished - 2012
Externally publishedYes
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X - San Francisco, CA, United States
Duration: Jan 21 2012Jan 23 2012

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Country/TerritoryUnited States
CitySan Francisco, CA


  • myofibrillogenesis
  • myosin filaments
  • on-stage incubator
  • sarcomere addition
  • second harmonic generation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Biomaterials


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