TY - JOUR
T1 - Lipogels for Encapsulation of Hydrophilic Proteins and Hydrophobic Small Molecules
AU - Homyak, Celia C.
AU - Fernandez, Ann
AU - Touve, Mollie A.
AU - Zhao, Bo
AU - Anson, Francesca
AU - Hardy, Jeanne A.
AU - Vachet, Richard W.
AU - Gianneschi, Nathan C.
AU - Ross, Jennifer L.
AU - Thayumanavan, S.
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2018/1/8
Y1 - 2018/1/8
N2 - Lipid-polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV-vis spectroscopy, fluorescence spectroscopy, and single-particle total internal reflection fluorescence (TIRF) microscopy. In addition, we confirmed that these dual-guest-loaded lipogels are stable in solution for several months. The simplicity of this complete aqueous formation and noncovalent dual-guest encapsulation holds potential as a tunable nanomaterial scaffold.
AB - Lipid-polymer hybrid materials have the potential to exhibit enhanced stability and loading capabilities in comparison to parent liposome or polymer materials. However, complexities lie in formulating and characterizing such complex nanomaterials. Here we describe a lipid-coated polymer gel (lipogel) formulated using a single-pot methodology, where self-assembling liposomes template a UV-curable polymer gel core. Using fluorescently labeled lipids, protein, and hydrophobic molecules, we characterized their formation, purification, stability, and encapsulation efficiency via common instrumentation methods such as dynamic light scattering (DLS), matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS), UV-vis spectroscopy, fluorescence spectroscopy, and single-particle total internal reflection fluorescence (TIRF) microscopy. In addition, we confirmed that these dual-guest-loaded lipogels are stable in solution for several months. The simplicity of this complete aqueous formation and noncovalent dual-guest encapsulation holds potential as a tunable nanomaterial scaffold.
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U2 - 10.1021/acs.biomac.7b01300
DO - 10.1021/acs.biomac.7b01300
M3 - Article
C2 - 29141403
AN - SCOPUS:85040308068
SN - 1525-7797
VL - 19
SP - 132
EP - 140
JO - Biomacromolecules
JF - Biomacromolecules
IS - 1
ER -