Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins

Carlos A. Castañeda, Emma K. Dixon, Olivier Walker, Apurva Chaturvedi, Mark A. Nakasone, Joseph E. Curtis, Megan R. Reed, Susan Krueger, T. Ashton Cropp, David Fushman

Research output: Research - peer-reviewArticle

  • 12 Citations

Abstract

Summary Polyubiquitination, a critical protein post-translational modification, signals for a diverse set of cellular events via the different isopeptide linkages formed between the C terminus of one ubiquitin (Ub) and the E-amine of K6, K11, K27, K29, K33, K48, or K63 of a second Ub. We assembled di-ubiquitins (Ub2) comprising every lysine linkage and examined them biochemically and structurally. Of these, K27-Ub2 is unique as it is not cleaved by most deubiquitinases. As this remains the only structurally uncharacterized lysine linkage, we comprehensively examined the structures and dynamics of K27-Ub2 using nuclear magnetic resonance, small-angle neutron scattering, and in silico ensemble modeling. Our structural data provide insights into the functional properties of K27-Ub2, in particular that K27-Ub2 may be specifically recognized by K48-selective receptor UBA2 domain from proteasomal shuttle protein hHR23a. Binding studies and mutagenesis confirmed this prediction, further highlighting structural/recognition versatility of polyubiquitins and the potential power of determining function from elucidation of conformational ensembles.

LanguageEnglish (US)
Pages423-436
Number of pages14
JournalStructure
Volume24
Issue number3
DOIs
StatePublished - Mar 1 2016

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Polyubiquitin
Ubiquitin
Lysine
Ubiquitins
Small Angle Scattering
Neutrons
Post Translational Protein Processing
Mutagenesis
Computer Simulation
Amines
Magnetic Resonance Spectroscopy
Proteins
Deubiquitinating Enzymes

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Castañeda, C. A., Dixon, E. K., Walker, O., Chaturvedi, A., Nakasone, M. A., Curtis, J. E., ... Fushman, D. (2016). Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. Structure, 24(3), 423-436. DOI: 10.1016/j.str.2016.01.007

Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. / Castañeda, Carlos A.; Dixon, Emma K.; Walker, Olivier; Chaturvedi, Apurva; Nakasone, Mark A.; Curtis, Joseph E.; Reed, Megan R.; Krueger, Susan; Cropp, T. Ashton; Fushman, David.

In: Structure, Vol. 24, No. 3, 01.03.2016, p. 423-436.

Research output: Research - peer-reviewArticle

Castañeda, CA, Dixon, EK, Walker, O, Chaturvedi, A, Nakasone, MA, Curtis, JE, Reed, MR, Krueger, S, Cropp, TA & Fushman, D 2016, 'Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins' Structure, vol 24, no. 3, pp. 423-436. DOI: 10.1016/j.str.2016.01.007
Castañeda CA, Dixon EK, Walker O, Chaturvedi A, Nakasone MA, Curtis JE et al. Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. Structure. 2016 Mar 1;24(3):423-436. Available from, DOI: 10.1016/j.str.2016.01.007
Castañeda, Carlos A. ; Dixon, Emma K. ; Walker, Olivier ; Chaturvedi, Apurva ; Nakasone, Mark A. ; Curtis, Joseph E. ; Reed, Megan R. ; Krueger, Susan ; Cropp, T. Ashton ; Fushman, David. / Linkage via K27 Bestows Ubiquitin Chains with Unique Properties among Polyubiquitins. In: Structure. 2016 ; Vol. 24, No. 3. pp. 423-436
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