Light-dependent hypersensitive response and resistance signaling against Turnip Crinkle Virus in Arabidopsis

A. C. Chandra-Shekara, Manisha Gupte, Duroy Navarre, Surabhi Raina, Ramesh Raina, Daniel Klessig, Pradeep Kachroo

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Resistance to Turnip Crinkle Virus (TCV) in Arabidopsis ecotype Dijon (Di)-17 is conferred by the resistance gene HRT and a recessive locus rrt. In Di-17, TCV elicits a hypersensitive response (HR), which is accompanied by increased expression of pathogenesis-related (PR) genes and high levels of salicylic acid (SA). We have previously shown that HRT-mediated resistance to TCV is dependent on SA-mediated signal transduction and that q2increased levels of SA confer enhanced resistance to TCV via upregulation of the HRT gene. Here we show that HRT-mediated HR and resistance are dependent on light. A dark treatment immediately following TCV inoculation suppressed HR, resistance and activation of the majority of the TCV-induced genes. However, the absence of light did not affect either TCV-induced elevated levels of free SA or the expression of HRT. Interestingly, in the dark, transgenic plants overexpressing HRT showed susceptibility, but overexpression of HRT coupled with high levels of endogenous SA resulted in pronounced resistance. Consistent with these results is the finding that exogenous application of SA prior to TCV inoculation partially overcame the requirement for light. Light was also required for N geneq3-mediated HR and resistance to Tobacco Mosaic Virus, suggesting that it is an important factor which may be generally required during defense signaling.

Original languageEnglish (US)
Pages (from-to)320-334
Number of pages15
JournalPlant Journal
Volume45
Issue number3
DOIs
StatePublished - Feb 2006

Fingerprint

Carmovirus
Turnip crinkle virus
hypersensitive response
Arabidopsis
Salicylic Acid
salicylic acid
Light
Genes
genes
Ecotype
Tobacco Mosaic Virus
light requirement
Tobacco mosaic virus
Genetically Modified Plants
ecotypes
signal transduction
transgenic plants
Signal Transduction
Up-Regulation
pathogenesis

Keywords

  • Arabidopsis
  • Defense
  • Light
  • Salicylic acid
  • Signaling
  • ssi2
  • Turnip Crinkle Virus

ASJC Scopus subject areas

  • Plant Science

Cite this

Light-dependent hypersensitive response and resistance signaling against Turnip Crinkle Virus in Arabidopsis. / Chandra-Shekara, A. C.; Gupte, Manisha; Navarre, Duroy; Raina, Surabhi; Raina, Ramesh; Klessig, Daniel; Kachroo, Pradeep.

In: Plant Journal, Vol. 45, No. 3, 02.2006, p. 320-334.

Research output: Contribution to journalArticle

Chandra-Shekara, A. C. ; Gupte, Manisha ; Navarre, Duroy ; Raina, Surabhi ; Raina, Ramesh ; Klessig, Daniel ; Kachroo, Pradeep. / Light-dependent hypersensitive response and resistance signaling against Turnip Crinkle Virus in Arabidopsis. In: Plant Journal. 2006 ; Vol. 45, No. 3. pp. 320-334.
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