Library Construction for next Generation Sequencing

Huseyin Kucuktas, Zhanjiang John Liu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations
Original languageEnglish (US)
Title of host publicationNext Generation Sequencing and Whole Genome Selection in Aquaculture
PublisherWiley Blackwell
Pages57-67
Number of pages11
ISBN (Print)9780813806372
DOIs
StatePublished - Dec 10 2010
Externally publishedYes

Keywords

  • DNA end repair, problems of DNA after fragmentation - need for end polishing
  • DNA fragmentation, first step of DNA sample preparation - generating fragments of various sizes
  • DNA fragmentation, through use of enzymatic fragmentation - as New England Biolabs (NEB) fragmentase
  • General procedures of library preparation - for next generation sequencing
  • Illumina sequencing platform, sample preparation - and sources of DNA or RNA and purpose of sequencing project
  • Indexed libraries, sequencing multiple samples in a single lane
  • Library construction for next generation sequencing
  • Sample preparation for Illumina sequencing
  • Sample preparation for sequencing multiplexed samples - using SOLiD sequencing
  • Sonication, shears DNA into small fragments - use of hydrodynamic force using a sonicator

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Kucuktas, H., & Liu, Z. J. (2010). Library Construction for next Generation Sequencing. In Next Generation Sequencing and Whole Genome Selection in Aquaculture (pp. 57-67). Wiley Blackwell. https://doi.org/10.1002/9780470958964.ch4