TY - CHAP
T1 - Library Construction for next Generation Sequencing
AU - Kucuktas, Huseyin
AU - Liu, Zhanjiang John
PY - 2010/12/10
Y1 - 2010/12/10
KW - DNA end repair, problems of DNA after fragmentation - need for end polishing
KW - DNA fragmentation, first step of DNA sample preparation - generating fragments of various sizes
KW - DNA fragmentation, through use of enzymatic fragmentation - as New England Biolabs (NEB) fragmentase
KW - General procedures of library preparation - for next generation sequencing
KW - Illumina sequencing platform, sample preparation - and sources of DNA or RNA and purpose of sequencing project
KW - Indexed libraries, sequencing multiple samples in a single lane
KW - Library construction for next generation sequencing
KW - Sample preparation for Illumina sequencing
KW - Sample preparation for sequencing multiplexed samples - using SOLiD sequencing
KW - Sonication, shears DNA into small fragments - use of hydrodynamic force using a sonicator
UR - http://www.scopus.com/inward/record.url?scp=84886165729&partnerID=8YFLogxK
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U2 - 10.1002/9780470958964.ch4
DO - 10.1002/9780470958964.ch4
M3 - Chapter
AN - SCOPUS:84886165729
SN - 9780813806372
SP - 57
EP - 67
BT - Next Generation Sequencing and Whole Genome Selection in Aquaculture
PB - Wiley Blackwell
ER -