Laser-guided cell micropatterning system

Russell K. Pirlo, Zhen Ma, Andrew Sweeney, Honghai Liu, Julie X. Yun, Xiang Peng, Xiaocong Yuan, George X. Guo, Bruce Z. Gao

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Employing optical force, our laser-guided cell micropatterning system, is capable of patterning different cell types onto and within standard cell research devices, including commercially available multielectrode arrays (MEAs) with glass culture rings, 35 mm Petri dishes, and microdevices microfabricated with polydimethylsiloxane on 22 mm 22 mm cover glasses. We discuss the theory of optical forces for generating laser guidance and the calculation of optimal beam characteristics for cell guidance. We describe the hardware design and software program for the cell patterning system. Finally, we demonstrate the capabilities of the system by (1) patterning neurons to form an arbitrary pattern, (2) patterning neurons onto the electrodes of a standard MEA, and (3) patterning and aligning adult cardiomyocytes in a polystyrene Petri dish.

Original languageEnglish (US)
Article number013708
JournalReview of Scientific Instruments
Volume82
Issue number1
DOIs
StatePublished - Jan 2011
Externally publishedYes

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ASJC Scopus subject areas

  • Instrumentation
  • Medicine(all)

Cite this

Pirlo, R. K., Ma, Z., Sweeney, A., Liu, H., Yun, J. X., Peng, X., Yuan, X., Guo, G. X., & Gao, B. Z. (2011). Laser-guided cell micropatterning system. Review of Scientific Instruments, 82(1), [013708]. https://doi.org/10.1063/1.3529919