Laser guidance in a microfluidic biochip

Wan Qin, Lucas Schmidt, Xiaoqi Yang, Lina Wei, Ting Huang, Julie X. Yuan, Zhen Ma, Xiang Peng, Xiaocong Yuan, Bruce Z. Gao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

In this study, we developed a microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and chick embryonic spinal cord neurons. The neurons we obtained from these two cell types have no difference in morphology as observed under a high-magnification microscope. However, when flowing in the microfluidic channel and simultaneously being laser-guided, the two cell types gained quite different guidance velocities under the same experimental conditions. The experimental results demonstrate that different cell types with the same morphology (e.g., size, shape, etc.) can be effectively distinguished from each other by measuring the difference of guidance velocities (the maximum flow velocities minus the initial flow velocities). This technique is expected to provide a new approach to high-throughput, label-free cell sorting with sensitivity.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems XI
DOIs
StatePublished - May 29 2013
Externally publishedYes
EventMicrofluidics, BioMEMS, and Medical Microsystems XI - San Francisco, CA, United States
Duration: Feb 3 2013Feb 5 2013

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8615
ISSN (Print)0277-786X

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems XI
CountryUnited States
CitySan Francisco, CA
Period2/3/132/5/13

Keywords

  • Cell sorting
  • laser guidance
  • microfluidics
  • optical force

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Qin, W., Schmidt, L., Yang, X., Wei, L., Huang, T., Yuan, J. X., Ma, Z., Peng, X., Yuan, X., & Gao, B. Z. (2013). Laser guidance in a microfluidic biochip. In Microfluidics, BioMEMS, and Medical Microsystems XI [86150S] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 8615). https://doi.org/10.1117/12.2003255