Laser-guidance based detection of cells with single-gene modification

Zhen Ma; Karen J.L. Burg; Yanzhang Wei; X. C. Yuan; Xiang Peng; Bruce Z. Gao

doi:10.1063/1.2938020

Laser-guidance based detection of cells with single-gene modification

Zhen Ma, Karen J.L. Burg, Yanzhang Wei, X. C. Yuan, Xiang Peng, Bruce Z. Gao

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

An optical method with single-gene sensitivity for cell detection based on laser guidance was explored. Guided by the optical force from a weakly focused laser beam, a cell will move along the laser axis. Cells with different properties experience different optical forces and thus guidance speeds. The guidance speeds of the TC-1 cell and its genetically modified counterpart with only one gene change, L-10 cell, were studied under the same conditions. The results demonstrated that this laser guidance-based speed-measurement method can precisely distinguish cells that differ by only one gene.

Original language	English (US)
Article number	213902
Journal	Applied Physics Letters
Volume	92
Issue number	21
DOIs	https://doi.org/10.1063/1.2938020
State	Published - 2008
Externally published	Yes

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

Access to Document

10.1063/1.2938020

Cite this

@article{997edba58b4240ad92b50ece57cbc520,

title = "Laser-guidance based detection of cells with single-gene modification",

abstract = "An optical method with single-gene sensitivity for cell detection based on laser guidance was explored. Guided by the optical force from a weakly focused laser beam, a cell will move along the laser axis. Cells with different properties experience different optical forces and thus guidance speeds. The guidance speeds of the TC-1 cell and its genetically modified counterpart with only one gene change, L-10 cell, were studied under the same conditions. The results demonstrated that this laser guidance-based speed-measurement method can precisely distinguish cells that differ by only one gene.",

author = "Zhen Ma and Burg, {Karen J.L.} and Yanzhang Wei and Yuan, {X. C.} and Xiang Peng and Gao, {Bruce Z.}",

note = "Funding Information: The research was partially supported by National Institutes of Health (SC INBRE Grant 2p20RR16461-05 and K25 award 1K25HL088262-010), Department of Defense Hope Award (BC044778), and a Ralph E. Powe Junior Faculty Enhancement Award. The authors thank Mr. Justin Raman for help in the design of the guidance chamber and Mr. Hari Kotturi and Ms. Cassie Gregory for their assistance in cell-sample preparation. X.C.Y. acknowledges National Natural Science Foundation of China for Grant No. 60778045.",

year = "2008",

doi = "10.1063/1.2938020",

language = "English (US)",

volume = "92",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

number = "21",

}

TY - JOUR

T1 - Laser-guidance based detection of cells with single-gene modification

AU - Ma, Zhen

AU - Burg, Karen J.L.

AU - Wei, Yanzhang

AU - Yuan, X. C.

AU - Peng, Xiang

AU - Gao, Bruce Z.

N1 - Funding Information: The research was partially supported by National Institutes of Health (SC INBRE Grant 2p20RR16461-05 and K25 award 1K25HL088262-010), Department of Defense Hope Award (BC044778), and a Ralph E. Powe Junior Faculty Enhancement Award. The authors thank Mr. Justin Raman for help in the design of the guidance chamber and Mr. Hari Kotturi and Ms. Cassie Gregory for their assistance in cell-sample preparation. X.C.Y. acknowledges National Natural Science Foundation of China for Grant No. 60778045.

PY - 2008

Y1 - 2008

N2 - An optical method with single-gene sensitivity for cell detection based on laser guidance was explored. Guided by the optical force from a weakly focused laser beam, a cell will move along the laser axis. Cells with different properties experience different optical forces and thus guidance speeds. The guidance speeds of the TC-1 cell and its genetically modified counterpart with only one gene change, L-10 cell, were studied under the same conditions. The results demonstrated that this laser guidance-based speed-measurement method can precisely distinguish cells that differ by only one gene.

AB - An optical method with single-gene sensitivity for cell detection based on laser guidance was explored. Guided by the optical force from a weakly focused laser beam, a cell will move along the laser axis. Cells with different properties experience different optical forces and thus guidance speeds. The guidance speeds of the TC-1 cell and its genetically modified counterpart with only one gene change, L-10 cell, were studied under the same conditions. The results demonstrated that this laser guidance-based speed-measurement method can precisely distinguish cells that differ by only one gene.

UR - http://www.scopus.com/inward/record.url?scp=44449127710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=44449127710&partnerID=8YFLogxK

U2 - 10.1063/1.2938020

DO - 10.1063/1.2938020

M3 - Article

AN - SCOPUS:44449127710

SN - 0003-6951

VL - 92

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 21

M1 - 213902

ER -

Laser-guidance based detection of cells with single-gene modification

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this