Abstract
Due to the extreme complexity of in vivo environments, our understanding of cellular functions and cell-cell interactions is heavily dependent on cell culture. To understand the biological mechanisms at the cellular level in cell culture, cell-patterning methods have been developed to mimic in vivo patterns of cellular organization. Unlike traditional cell patterning techniques, which are not designed to pattern small numbers of cells with the accuracy desired for systematic cell-cell interaction studies, laser guidance-based cell micropatterning uses optical force to capture a cell and guide it to a specific location on a variety of substrates. In this chapter, we will discuss theories on optical force, demonstrate numerical simulations, and describe the design and implementation of an actual micropatterning system. Three cell patterns will be presented: a single-cell array with high spatial resolution (less than 1∈μm), alignment of rod-shape adult cardiomyocytes, and neuronal networks with defined connectivity and single-cell resolution on a microelectrode array.
| Original language | English (US) |
|---|---|
| Title of host publication | Cell and Organ Printing |
| Publisher | Springer Netherlands |
| Pages | 137-159 |
| Number of pages | 23 |
| ISBN (Print) | 9789048191444 |
| DOIs | |
| State | Published - 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Laser guidance-based cell micropatterning. / Ma, Zhen; Pirlo, Russell K.; Yun, Julie X.; Peng, Xiang; Yuan, Xiaocong; Gao, Bruce Z.
Cell and Organ Printing. Springer Netherlands, 2010. p. 137-159.Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Laser guidance-based cell micropatterning
AU - Ma, Zhen
AU - Pirlo, Russell K.
AU - Yun, Julie X.
AU - Peng, Xiang
AU - Yuan, Xiaocong
AU - Gao, Bruce Z.
PY - 2010
Y1 - 2010
N2 - Due to the extreme complexity of in vivo environments, our understanding of cellular functions and cell-cell interactions is heavily dependent on cell culture. To understand the biological mechanisms at the cellular level in cell culture, cell-patterning methods have been developed to mimic in vivo patterns of cellular organization. Unlike traditional cell patterning techniques, which are not designed to pattern small numbers of cells with the accuracy desired for systematic cell-cell interaction studies, laser guidance-based cell micropatterning uses optical force to capture a cell and guide it to a specific location on a variety of substrates. In this chapter, we will discuss theories on optical force, demonstrate numerical simulations, and describe the design and implementation of an actual micropatterning system. Three cell patterns will be presented: a single-cell array with high spatial resolution (less than 1∈μm), alignment of rod-shape adult cardiomyocytes, and neuronal networks with defined connectivity and single-cell resolution on a microelectrode array.
AB - Due to the extreme complexity of in vivo environments, our understanding of cellular functions and cell-cell interactions is heavily dependent on cell culture. To understand the biological mechanisms at the cellular level in cell culture, cell-patterning methods have been developed to mimic in vivo patterns of cellular organization. Unlike traditional cell patterning techniques, which are not designed to pattern small numbers of cells with the accuracy desired for systematic cell-cell interaction studies, laser guidance-based cell micropatterning uses optical force to capture a cell and guide it to a specific location on a variety of substrates. In this chapter, we will discuss theories on optical force, demonstrate numerical simulations, and describe the design and implementation of an actual micropatterning system. Three cell patterns will be presented: a single-cell array with high spatial resolution (less than 1∈μm), alignment of rod-shape adult cardiomyocytes, and neuronal networks with defined connectivity and single-cell resolution on a microelectrode array.
UR - http://www.scopus.com/inward/record.url?scp=84882278075&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882278075&partnerID=8YFLogxK
U2 - 10.1007/978-90-481-9145-1_8
DO - 10.1007/978-90-481-9145-1_8
M3 - Chapter
AN - SCOPUS:84882278075
SN - 9789048191444
SP - 137
EP - 159
BT - Cell and Organ Printing
PB - Springer Netherlands
ER -