Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning

Zhen Ma, Russell K. Pirlo, Qin Wan, Julie X. Yun, Xiaocong Yuan, Peng Xiang, Thomas K. Borg, Bruce Z. Gao

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Cell patterning methods enable researchers to control specific homotypic and heterotypic contact-mediated cell-cell and cell-ECM interactions and to impose defined cell and tissue geometries. To micropattern individual cells to specific points on a substrate with high spatial resolution, we have developed a cell deposition microscope based on the laser-guidance technique. We discuss the theory of optical forces for generating laser guidance and the optimization of the optical configuration (NA ≈ 0.1) to manipulate cells with high speed in three dimensions. Our cell deposition microscope is capable of patterning different cell types onto and within standard cell research devices and providing on-stage incubation for long-term cell culturing. Using this cell deposition microscope, rat mesenchymal stem cells from bone marrow were micropatterned with cardiomyocytes into a substrate microfabricated with polydimethylsiloxane on a 22 mm × 22 mm coverglass to form a single-cell coculturing microenvironment, and their electrophysiological property changes were investigated during the coculturing days.

Original languageEnglish (US)
Article number034107
JournalBiofabrication
Volume3
Issue number3
DOIs
StatePublished - Sep 2011

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

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    Ma, Z., Pirlo, R. K., Wan, Q., Yun, J. X., Yuan, X., Xiang, P., Borg, T. K., & Gao, B. Z. (2011). Laser-guidance-based cell deposition microscope for heterotypic single-cell micropatterning. Biofabrication, 3(3), [034107]. https://doi.org/10.1088/1758-5082/3/3/034107