Kinetics of Membrane Protein-Detergent Interactions Depend on Protein Electrostatics

Aaron J. Wolfe, Jack F. Gugel, Min Chen, Liviu Movileanu

Research output: Contribution to journalArticle

Abstract

Interactions of a membrane protein with a detergent micelle represent a fundamental process with practical implications in structural and chemical biology. Quantitative assessment of the kinetics of protein-detergent complex (PDC) interactions has always been challenged by complicated behavior of both membrane proteins and solubilizing detergents in aqueous phase. Here, we show the kinetic reads of the desorption of maltoside-containing detergents from β-barrel membrane proteins. Using steady-state fluorescence polarization (FP) anisotropy measurements, we recorded real-time, specific signatures of the PDC interactions. The results of these measurements were used to infer the model-dependent rate constants of association and dissociation of the proteomicelles. Remarkably, the kinetics of the PDC interactions depend on the overall protein charge despite the nonionic nature of the detergent monomers. In the future, this approach might be employed for high-throughput screening of kinetic fingerprints of different membrane proteins stabilized in micelles that contain mixtures of various detergents.

Original languageEnglish (US)
Pages (from-to)9471-9481
Number of pages11
JournalJournal of Physical Chemistry B
Volume122
Issue number41
DOIs
StatePublished - Oct 18 2018

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detergents
Detergents
Electrostatics
Membrane Proteins
electrostatics
membranes
proteins
Proteins
Membranes
Kinetics
kinetics
interactions
Micelles
micelles
biology
Rate constants
Desorption
Screening
Anisotropy
screening

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Surfaces, Coatings and Films
  • Materials Chemistry

Cite this

Kinetics of Membrane Protein-Detergent Interactions Depend on Protein Electrostatics. / Wolfe, Aaron J.; Gugel, Jack F.; Chen, Min; Movileanu, Liviu.

In: Journal of Physical Chemistry B, Vol. 122, No. 41, 18.10.2018, p. 9471-9481.

Research output: Contribution to journalArticle

Wolfe, Aaron J. ; Gugel, Jack F. ; Chen, Min ; Movileanu, Liviu. / Kinetics of Membrane Protein-Detergent Interactions Depend on Protein Electrostatics. In: Journal of Physical Chemistry B. 2018 ; Vol. 122, No. 41. pp. 9471-9481.
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