JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time

Aditya Dutta, Pratibha Choudhary, Julie Caruana, Ramesh Raina

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Histone methylation is known to dynamically regulate diverse developmental and physiological processes. Histone methyl marks are written by methyltransferases and erased by demethylases, and result in modification of chromatin structure to repress or activate transcription. However, little is known about how histone methylation may regulate defense mechanisms and flowering time in plants. Here we report characterization of JmjC DOMAIN-CONTAINING PROTEIN 27 (JMJ27), an Arabidopsis JHDM2 (JmjC domain-containing histone demethylase 2) family protein, which modulates defense against pathogens and flowering time. JMJ27 is a nuclear protein containing a zinc-finger motif and a catalytic JmjC domain with conserved Fe(II) and α-ketoglutarate binding sites, and displays H3K9me1/2 demethylase activity both in vitro and in vivo. JMJ27 is induced in response to virulent Pseudomonas syringae pathogens and is required for resistance against these pathogens. JMJ27 is a negative modulator of WRKY25 (a repressor of defense) and a positive modulator of several pathogenesis-related (PR) proteins. Additionally, loss of JMJ27 function leads to early flowering. JMJ27 negatively modulates the major flowering regulator CONSTANS (CO) and positively modulates FLOWERING LOCUS C (FLC). Taken together, our results indicate that JMJ27 functions as a histone demethylase to modulate both physiological (defense) and developmental (flowering time) processes in Arabidopsis.

Original languageEnglish (US)
Pages (from-to)1015-1028
Number of pages14
JournalPlant Journal
Volume91
Issue number6
DOIs
StatePublished - Sep 1 2017

Fingerprint

Histone Demethylases
Pseudomonas syringae
Arabidopsis
histones
flowering
Jumonji Domain-Containing Histone Demethylases
Histones
Methylation
Histone Code
Physiological Phenomena
methylation
Zinc Fingers
Methyltransferases
pathogens
Nuclear Proteins
Chromatin
Catalytic Domain
Proteins
Binding Sites
pathogenesis-related proteins

Keywords

  • Arabidopsis thaliana
  • bacterial pathogen
  • chromatin modification
  • defense signaling
  • flowering time
  • H3K9
  • histone demethylase
  • JMJ27
  • jumonji-domain

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time. / Dutta, Aditya; Choudhary, Pratibha; Caruana, Julie; Raina, Ramesh.

In: Plant Journal, Vol. 91, No. 6, 01.09.2017, p. 1015-1028.

Research output: Contribution to journalArticle

Dutta, Aditya ; Choudhary, Pratibha ; Caruana, Julie ; Raina, Ramesh. / JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time. In: Plant Journal. 2017 ; Vol. 91, No. 6. pp. 1015-1028.
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