JAK and STAT members in channel catfish: Identification, phylogenetic analysis and expression profiling after Edwardsiella ictaluri infection

Yulin Jin, Tao Zhou, Ning Li, Shikai Liu, Xiaoyan Xu, Ying Pan, Suxu Tan, Huitong Shi, Yujia Yang, Zihao Yuan, Wenwen Wang, Jian Luo, Dongya Gao, Rex Dunham, Zhanjian "John" Liu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is one of the main pleiotropic cascades used to transmit information from extracellular receptors to the nucleus, which results in DNA transcription and expression of genes involved in immunity, proliferation, differentiation, migration, apoptosis, and cell survival. Members of JAK family and STAT family have been extensively studied in different mammalian species because of their important roles in innate and adaptive immune responses. However, they have not been systematically studied among teleost fish species. In this study, five JAK family members and eight STAT family members were identified and characterized from channel catfish. Phylogenetic analysis was conducted to properly annotate these genes. Syntenic analysis was also conducted to establish orthology, and confirm the results from phylogenetic analysis. Compared to mammals, more members of the JAK and STAT family were identified in channel catfish genome. Expression of JAK and STAT family members was detected in healthy catfish tissues, but was induced in gill, liver, and intestine after bacterial challenge. Notably, the significant upregulation of STAT1b gene in catfish liver, gill and intestine after Edwardsiella ictaluri infection supported the notion that high STAT1 expression are involved in defense against pathogens. Collectively, the increased expression of JAK and STAT members in tested tissues suggested their crucial function in defending the host against pathogen invasion.

Original languageEnglish (US)
Pages (from-to)334-341
Number of pages8
JournalDevelopmental and Comparative Immunology
Volume81
DOIs
StatePublished - Apr 1 2018

Fingerprint

Edwardsiella ictaluri
Ictaluridae
Infection
Catfishes
Intestines
Janus Kinases
Liver
Adaptive Immunity
Transducers
Innate Immunity
Genes
Mammals
Immunity
Cell Survival
Fishes
Up-Regulation
Genome
Apoptosis
Gene Expression
DNA

Keywords

  • Catfish
  • Edwardsiella ictaluri
  • Immunity
  • JAK
  • JAK/STAT signaling pathway
  • STAT

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

JAK and STAT members in channel catfish : Identification, phylogenetic analysis and expression profiling after Edwardsiella ictaluri infection. / Jin, Yulin; Zhou, Tao; Li, Ning; Liu, Shikai; Xu, Xiaoyan; Pan, Ying; Tan, Suxu; Shi, Huitong; Yang, Yujia; Yuan, Zihao; Wang, Wenwen; Luo, Jian; Gao, Dongya; Dunham, Rex; Liu, Zhanjian "John".

In: Developmental and Comparative Immunology, Vol. 81, 01.04.2018, p. 334-341.

Research output: Contribution to journalArticle

Jin, Yulin ; Zhou, Tao ; Li, Ning ; Liu, Shikai ; Xu, Xiaoyan ; Pan, Ying ; Tan, Suxu ; Shi, Huitong ; Yang, Yujia ; Yuan, Zihao ; Wang, Wenwen ; Luo, Jian ; Gao, Dongya ; Dunham, Rex ; Liu, Zhanjian "John". / JAK and STAT members in channel catfish : Identification, phylogenetic analysis and expression profiling after Edwardsiella ictaluri infection. In: Developmental and Comparative Immunology. 2018 ; Vol. 81. pp. 334-341.
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