Isolation and structural properties of cytoplasmic glycerol-3-phosphate dehydrogenase from rat liver

Thomas P. Fondy, Kenneth J. Herwig, Susan J. Sollohub, Diane Barr Rutherford

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Cytoplasmic, NAD-linked l-glycerol-3-P dehydrogenase (EC 1.1.1.8) was shown to exist as a single major isoenzymic from in the livers of 120-200-g rats when fresh homogenates were examined by polyacrylamide gel isoelectric focusing. The enzyme was isolated in apparently homogenous form and the amino acid composition established. The molecular weight estimated by gel filtration was 63.000±6,000.

Original languageEnglish (US)
Pages (from-to)583-590
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume145
Issue number2
DOIs
StatePublished - Aug 1971

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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