TY - JOUR
T1 - Isolation and characterization of βact in gene of Carp (cyprinus carpio)
AU - Liu, Zhanjiang
AU - Zhu, Zuoyan
AU - Roberg, Kevin
AU - Faras, Anthony
AU - Guise, Kevin
AU - Kapuscinski, Anne R.
AU - Hacketptt, Perry B.
N1 - Funding Information:
We thank Ling He, Yuefeng Xie, Guohua Li and Kesheng Xu (Institute of Hydrobiology, Wuhan, People’s Republic of China) for help in the preparation of the carp genomic library; Steve Hughes for providing his chicken 8-actin gene clone; Aris Moustakas and Boaz Moav for discussions and reading the manuscript; Peter Saurugger and Darrin Johnson (University of Minnesota Molecular Biology Computing Center) for help with the lntelligenetics Programs; Kris Kirkeby and Toots McTavish for preparing the figures; and Carol Heiser for typing the manuscript. This work was supported by grants from NIH and the Minnesota Sea Grant College Program, supported by the NOAA Office of Sea Grant, DOC to P.H. Z.L. was supported by a stipend from Minnesota Sea Grant; Z.Z. was supported by a University of Minnesota Hill Visiting Professorship. The sequence has been deposited in the GenBank database under the accession number M241134.
PY - 1990
Y1 - 1990
N2 - A βactin gene of carp (Cyprinus carpio) was isolated from a genomic EMBL3 library. The nucleotide sequence of the gene indicates six exons spanning 3.6 kb. Southern blot hybridization of restriction endonuclease digests of carp genomic DNA indicate that there are two copies of the βactin isotype and several other species of actin genes. The transcriptional start site is 85 bp and 24 bp downstream respectively from consensus CCAAT and TATA promoter elements. The organization of the carp βactin gene is identical to that of chicken, human, and rat genes in terms of size, exon/intron locations and junctions and in having a translationally silent first exon. The fish gene is 90% and 99% conserved at the nucleotide and amino acid levels, respectively, with land vertebrate βactin genes. Northern blot analysis of βactin gene expression indicated that the gene is highly expressed in brain, less so in muscle, and much less so in liver cells. The putative βactin proximal promoter of carp, identified by the conservation of known actin regulatory sequences, is transcriptionally active in both mammalian and piscine cells.
AB - A βactin gene of carp (Cyprinus carpio) was isolated from a genomic EMBL3 library. The nucleotide sequence of the gene indicates six exons spanning 3.6 kb. Southern blot hybridization of restriction endonuclease digests of carp genomic DNA indicate that there are two copies of the βactin isotype and several other species of actin genes. The transcriptional start site is 85 bp and 24 bp downstream respectively from consensus CCAAT and TATA promoter elements. The organization of the carp βactin gene is identical to that of chicken, human, and rat genes in terms of size, exon/intron locations and junctions and in having a translationally silent first exon. The fish gene is 90% and 99% conserved at the nucleotide and amino acid levels, respectively, with land vertebrate βactin genes. Northern blot analysis of βactin gene expression indicated that the gene is highly expressed in brain, less so in muscle, and much less so in liver cells. The putative βactin proximal promoter of carp, identified by the conservation of known actin regulatory sequences, is transcriptionally active in both mammalian and piscine cells.
KW - Evolutionary conservation
KW - Genomic library
KW - Mapping
KW - Promoter
KW - Tissue-specific expression
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U2 - 10.3109/10425179009016040
DO - 10.3109/10425179009016040
M3 - Article
C2 - 2134183
AN - SCOPUS:0025524687
SN - 1940-1736
VL - 1
SP - 125
EP - 136
JO - DNA Sequence - Journal of DNA Sequencing and Mapping
JF - DNA Sequence - Journal of DNA Sequencing and Mapping
IS - 2
ER -