Invited review: Microtubule severing enzymes couple atpase activity with tubulin GTPase spring loading

Megan E. Bailey, Nan Jiang, Ruxandra I. Dima, Jennifer L. Ross

Research output: Contribution to journalReview article

10 Scopus citations

Abstract

Microtubules are amazing filaments made of GTPase enzymes that store energy used for their own self-destruction to cause a stochastically driven dynamics called dynamic instability. Dynamic instability can be reproduced in vitro with purified tubulin, but the dynamics do not mimic that observed in cells. This is because stabilizers and destabilizers act to alter microtubule dynamics. One interesting and understudied class of destabilizers consists of the microtubule-severing enzymes from the ATPases Associated with various cellular Activities (AAA+) family of ATP-enzymes. Here we review current knowledge about GTP-driven microtubule dynamics and how that couples to ATP-driven destabilization by severing enzymes. We present a list of challenges regarding the mechanism of severing, which require development of experimental and modeling approaches to shed light as to how severing enzymes can act to regulate microtubule dynamics in cells.

Original languageEnglish (US)
Pages (from-to)547-556
Number of pages10
JournalBiopolymers
Volume105
Issue number8
DOIs
StatePublished - Aug 1 2016
Externally publishedYes

Keywords

  • katanin
  • microtubule
  • microtubule-severing enzyme
  • spastin
  • tubulin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

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