Interrogating single proteins through nanopores: challenges and opportunities

Research output: Contribution to journalReview articlepeer-review

153 Scopus citations


A single nanopore represents an amazingly versatile single-molecule probe that can be employed to reveal several important features of polypeptides, such as their folding state, backbone flexibility, mechanical stability, binding affinity to other interacting ligands and enzymatic activity. Moreover, groundwork in this area using engineered protein nanopores has demonstrated new opportunities for discovering the biophysical rules that govern the transport of proteins through transmembrane protein pores. In this review, I summarize the current knowledge in the field and discuss how nanopore probe techniques will provide a new generation of research tools in nanomedicine for quantitatively examining the details of complex recognition and, furthermore, will represent a crucial step in designing other pore-based nanostructures and high-throughput devices for molecular biomedical diagnosis.

Original languageEnglish (US)
Pages (from-to)333-341
Number of pages9
JournalTrends in Biotechnology
Issue number6
StatePublished - Jun 2009

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering


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