Interleukin-1β protects neurons against oxidant-induced injury via the promotion of astrocyte glutathione production

Twinkle Chowdhury, Matthew F. Allen, Trista L. Thorn, Yan He, Sandra Hewett

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Interleukin-1β (IL-1β), a key cytokine that drives neuroinflammation in the Central Nervous System (CNS), is enhanced in many neurological diseases/disorders. Although IL-1β contributes to and/or sustains pathophysiological processes in the CNS, we recently demonstrated that IL-1β can protect cortical astrocytes from oxidant injury in a glutathione (GSH)-dependent manner. To test whether IL-1β could similarly protect neurons against oxidant stress, near pure neuronal cultures or mixed cortical cell cultures containing neurons and astrocytes were exposed to the organic peroxide, tert-butyl hydroperoxide (t-BOOH), following treatment with IL-1β or its vehicle. Neurons and astrocytes in mixed cultures, but not pure neurons, were significantly protected from the toxicity of t-BOOH following treatment with IL-1β in association with enhanced GSH production/release. IL-1β failed to increase the GSH levels or to provide protection against t-BOOH toxicity in chimeric mixed cultures consisting of IL-1R1+/+ neurons plated on top of IL-1R1−/− astrocytes. The attenuation of GSH release via block of multidrug resistance-associated protein 1 (MRP1) transport also abrogated the protective effect of IL-1β. These protective effects were not strictly an in vitro phenomenon as we found an increased striatal vulnerability to 3-nitropropionic acid-mediated oxidative stress in IL-1R1 null mice. Overall, our data indicate that IL-1β protects neurons against oxidant injury and that this likely occurs in a non-cell-autonomous manner that relies on an increase in astrocyte GSH production and release.

Original languageEnglish (US)
Article number100
JournalAntioxidants
Volume7
Issue number8
DOIs
StatePublished - Aug 1 2018

Fingerprint

Interleukin-1
Oxidants
Astrocytes
Neurons
Glutathione
Wounds and Injuries
Neurology
Cell culture
Toxicity
Central Nervous System
tert-Butylhydroperoxide
Corpus Striatum
Oxidative stress
Peroxides
Protein Transport
Nervous System Diseases
Oxidative Stress
Cell Culture Techniques
Association reactions
Cytokines

Keywords

  • Antioxidant
  • Astrocyte
  • Glutathione
  • Interleukin 1β
  • Neuroinflammation
  • Neuronal injury
  • Neuroprotection
  • Non-cell-autonomous protection

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Interleukin-1β protects neurons against oxidant-induced injury via the promotion of astrocyte glutathione production. / Chowdhury, Twinkle; Allen, Matthew F.; Thorn, Trista L.; He, Yan; Hewett, Sandra.

In: Antioxidants, Vol. 7, No. 8, 100, 01.08.2018.

Research output: Contribution to journalArticle

Chowdhury, Twinkle ; Allen, Matthew F. ; Thorn, Trista L. ; He, Yan ; Hewett, Sandra. / Interleukin-1β protects neurons against oxidant-induced injury via the promotion of astrocyte glutathione production. In: Antioxidants. 2018 ; Vol. 7, No. 8.
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