An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1β (IL1β) plus interferon-γ (INFγ) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-l-arginine and the protein synthesis inhibitor cycloheximide.
- Nitric oxide synthase
ASJC Scopus subject areas