Interferon-γ and interleukin-1β induce nitric oxide formation from primary mouse astrocytes

Sandra J. Hewett; John A. Corbett; Michael L. McDaniel; Dennis W. Choi

doi:10.1016/0304-3940(93)90898-U

Interferon-γ and interleukin-1β induce nitric oxide formation from primary mouse astrocytes

Sandra J. Hewett, John A. Corbett, Michael L. McDaniel, Dennis W. Choi

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1β (IL1β) plus interferon-γ (INFγ) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor N^G-nitro-l-arginine and the protein synthesis inhibitor cycloheximide.

Original language	English (US)
Pages (from-to)	229-232
Number of pages	4
Journal	Neuroscience Letters
Volume	164
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90898-U
State	Published - Dec 24 1993
Externally published	Yes

Keywords

Astrocyte
Cytokine
Immunoprecipitation
Mouse
Nitric oxide synthase
Nitrite

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(93)90898-U

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title = "Interferon-γ and interleukin-1β induce nitric oxide formation from primary mouse astrocytes",

abstract = "An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1β (IL1β) plus interferon-γ (INFγ) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-l-arginine and the protein synthesis inhibitor cycloheximide.",

keywords = "Astrocyte, Cytokine, Immunoprecipitation, Mouse, Nitric oxide synthase, Nitrite",

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AU - Hewett, Sandra J.

AU - Corbett, John A.

AU - McDaniel, Michael L.

AU - Choi, Dennis W.

PY - 1993/12/24

Y1 - 1993/12/24

N2 - An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1β (IL1β) plus interferon-γ (INFγ) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-l-arginine and the protein synthesis inhibitor cycloheximide.

AB - An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1β (IL1β) plus interferon-γ (INFγ) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-l-arginine and the protein synthesis inhibitor cycloheximide.

KW - Astrocyte

KW - Cytokine

KW - Immunoprecipitation

KW - Mouse

KW - Nitric oxide synthase

KW - Nitrite

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JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Interferon-γ and interleukin-1β induce nitric oxide formation from primary mouse astrocytes

Abstract

Keywords

ASJC Scopus subject areas

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