Interferon-γ and interleukin-1β induce nitric oxide formation from primary mouse astrocytes

Sandra J. Hewett, John A. Corbett, Michael L. McDaniel, Dennis W. Choi

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1β (IL1β) plus interferon-γ (INFγ) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor NG-nitro-l-arginine and the protein synthesis inhibitor cycloheximide.

Original languageEnglish (US)
Pages (from-to)229-232
Number of pages4
JournalNeuroscience Letters
Volume164
Issue number1-2
DOIs
StatePublished - Dec 24 1993
Externally publishedYes

Keywords

  • Astrocyte
  • Cytokine
  • Immunoprecipitation
  • Mouse
  • Nitric oxide synthase
  • Nitrite

ASJC Scopus subject areas

  • Neuroscience(all)

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