Inhibition of nitric oxide formation does not protect murine cortical cell cultures from N-methyl-d-aspartate neurotoxicity

Sandra J. Hewett, John A. Corbett, Michael L. McDaniel, Dennis W. Choi

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

We examined the role of nitric oxide in N-methyl-d-aspartate (NMDA) receptor-mediated neurotoxicity in rat and mouse primary cortical cell cultures. In rat and mouse cultures, the NO synthase inhibitor, NG-Nitro-l-arginine, blocked cGMP formation but not neuronal cell death following a 5-10 min exposure to 300-500 μM NMDA. NG-Monomethyl-l-arginine was also unable to prevent neuronal death. In contrast, the non-competitive NMDA receptor antagonist, dextrophan, prevented both cGMP formation and cell death. While other data suggest that the synthesis of nitric oxide can mediate NMDA receptor-mediated neurotoxicity, present results suggest that such synthesis is not necessarily required.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalBrain Research
Volume625
Issue number2
DOIs
StatePublished - Oct 22 1993
Externally publishedYes

Keywords

  • Cell culture
  • Cortex
  • N-Nitro-l-arginine
  • NMDA
  • Neuronal damage
  • Nitric oxide
  • cGMP

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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