Inhibition of Candida albicans growth by brominated furanones

Miao Duo; Mi Zhang; Yan Yeung Luk; Dacheng Ren

doi:10.1007/s00253-009-2174-6

Inhibition of Candida albicans growth by brominated furanones

Miao Duo, Mi Zhang, Yan Yeung Luk, Dacheng Ren

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Candida albicans is the most virulent Candida species of medical importance, which presents a great threat to immunocompromised individuals such as HIV patients. Currently, there are only four classes of antifungal agents available for treating fungal infections: azoles, polyenes, pyrimidines, and echinocandins. The fast spread of multidrug resistant C. albicans strains has increased the demand for new antifungal drugs. In this study, we demonstrate the antifungal activity of brominated furanones on C. albicans. Studying the structure and activity of this class of furanones reveals that the exocyclic vinyl bromide conjugated with the carbonyl group is the most important structural element for fungal inhibition. Furthermore, gene expression analysis using DNA microarrays showed that 3 μg/mL of 4-bromo-5Z-(bromomethylene)-3- butylfuran-2-one (BF1) upregulated 32 C. albicans genes with functions of stress response, NADPH dehydrogenation, and small-molecule transport, and repressed 21 genes involved mainly in cell-wall maintenance. Interestingly, only a small overlap is observed between the gene expression changes caused by the representative brominated furanone (BF1) in this study and other antifungal drugs reported in literature. This result suggests that brominated furanones and other antifungal drugs may target different fungal proteins or genes. The existence of such new targets provides an opportunity for developing new agents to control fungal pathogens which are resistant to currently available drugs.

Original language	English (US)
Pages (from-to)	1551-1563
Number of pages	13
Journal	Applied Microbiology and Biotechnology
Volume	85
Issue number	5
DOIs	https://doi.org/10.1007/s00253-009-2174-6
State	Published - Feb 2010

Keywords

Brominated furanone
Candida albicans
DNA microarray
Growth inhibition

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

Access to Document

10.1007/s00253-009-2174-6

Cite this

@article{26c39ece04a84d92853789f3fe021e5b,

title = "Inhibition of Candida albicans growth by brominated furanones",

abstract = "Candida albicans is the most virulent Candida species of medical importance, which presents a great threat to immunocompromised individuals such as HIV patients. Currently, there are only four classes of antifungal agents available for treating fungal infections: azoles, polyenes, pyrimidines, and echinocandins. The fast spread of multidrug resistant C. albicans strains has increased the demand for new antifungal drugs. In this study, we demonstrate the antifungal activity of brominated furanones on C. albicans. Studying the structure and activity of this class of furanones reveals that the exocyclic vinyl bromide conjugated with the carbonyl group is the most important structural element for fungal inhibition. Furthermore, gene expression analysis using DNA microarrays showed that 3 μg/mL of 4-bromo-5Z-(bromomethylene)-3- butylfuran-2-one (BF1) upregulated 32 C. albicans genes with functions of stress response, NADPH dehydrogenation, and small-molecule transport, and repressed 21 genes involved mainly in cell-wall maintenance. Interestingly, only a small overlap is observed between the gene expression changes caused by the representative brominated furanone (BF1) in this study and other antifungal drugs reported in literature. This result suggests that brominated furanones and other antifungal drugs may target different fungal proteins or genes. The existence of such new targets provides an opportunity for developing new agents to control fungal pathogens which are resistant to currently available drugs.",

keywords = "Brominated furanone, Candida albicans, DNA microarray, Growth inhibition",

author = "Miao Duo and Mi Zhang and Luk, {Yan Yeung} and Dacheng Ren",

note = "Funding Information: Acknowledgements The authors are grateful to Syracuse University and Syracuse Center of Excellence (under the EPA grant X-83232501-0) for financial support. We also thank Dr. Sean Palecek (University of Wisconsin-Madison) for the strain of C. albicans SC3415, and Dr. Andre Nantel (National Research Council of Canada) for helping with DNA microarray analysis. We appreciate Drs. James H. Henderson and Shelley Kummer (Syracuse University) for suggestions on reverse transcription as well as Dr. Michael S. Cosgrove (Syracuse University) for access to a dark room.",

year = "2010",

month = feb,

doi = "10.1007/s00253-009-2174-6",

language = "English (US)",

volume = "85",

pages = "1551--1563",

journal = "Applied Microbiology and Biotechnology",

issn = "0175-7598",

publisher = "Springer Verlag",

number = "5",

}

TY - JOUR

T1 - Inhibition of Candida albicans growth by brominated furanones

AU - Duo, Miao

AU - Zhang, Mi

AU - Luk, Yan Yeung

AU - Ren, Dacheng

N1 - Funding Information: Acknowledgements The authors are grateful to Syracuse University and Syracuse Center of Excellence (under the EPA grant X-83232501-0) for financial support. We also thank Dr. Sean Palecek (University of Wisconsin-Madison) for the strain of C. albicans SC3415, and Dr. Andre Nantel (National Research Council of Canada) for helping with DNA microarray analysis. We appreciate Drs. James H. Henderson and Shelley Kummer (Syracuse University) for suggestions on reverse transcription as well as Dr. Michael S. Cosgrove (Syracuse University) for access to a dark room.

PY - 2010/2

Y1 - 2010/2

N2 - Candida albicans is the most virulent Candida species of medical importance, which presents a great threat to immunocompromised individuals such as HIV patients. Currently, there are only four classes of antifungal agents available for treating fungal infections: azoles, polyenes, pyrimidines, and echinocandins. The fast spread of multidrug resistant C. albicans strains has increased the demand for new antifungal drugs. In this study, we demonstrate the antifungal activity of brominated furanones on C. albicans. Studying the structure and activity of this class of furanones reveals that the exocyclic vinyl bromide conjugated with the carbonyl group is the most important structural element for fungal inhibition. Furthermore, gene expression analysis using DNA microarrays showed that 3 μg/mL of 4-bromo-5Z-(bromomethylene)-3- butylfuran-2-one (BF1) upregulated 32 C. albicans genes with functions of stress response, NADPH dehydrogenation, and small-molecule transport, and repressed 21 genes involved mainly in cell-wall maintenance. Interestingly, only a small overlap is observed between the gene expression changes caused by the representative brominated furanone (BF1) in this study and other antifungal drugs reported in literature. This result suggests that brominated furanones and other antifungal drugs may target different fungal proteins or genes. The existence of such new targets provides an opportunity for developing new agents to control fungal pathogens which are resistant to currently available drugs.

AB - Candida albicans is the most virulent Candida species of medical importance, which presents a great threat to immunocompromised individuals such as HIV patients. Currently, there are only four classes of antifungal agents available for treating fungal infections: azoles, polyenes, pyrimidines, and echinocandins. The fast spread of multidrug resistant C. albicans strains has increased the demand for new antifungal drugs. In this study, we demonstrate the antifungal activity of brominated furanones on C. albicans. Studying the structure and activity of this class of furanones reveals that the exocyclic vinyl bromide conjugated with the carbonyl group is the most important structural element for fungal inhibition. Furthermore, gene expression analysis using DNA microarrays showed that 3 μg/mL of 4-bromo-5Z-(bromomethylene)-3- butylfuran-2-one (BF1) upregulated 32 C. albicans genes with functions of stress response, NADPH dehydrogenation, and small-molecule transport, and repressed 21 genes involved mainly in cell-wall maintenance. Interestingly, only a small overlap is observed between the gene expression changes caused by the representative brominated furanone (BF1) in this study and other antifungal drugs reported in literature. This result suggests that brominated furanones and other antifungal drugs may target different fungal proteins or genes. The existence of such new targets provides an opportunity for developing new agents to control fungal pathogens which are resistant to currently available drugs.

KW - Brominated furanone

KW - Candida albicans

KW - DNA microarray

KW - Growth inhibition

UR - http://www.scopus.com/inward/record.url?scp=76649085215&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76649085215&partnerID=8YFLogxK

U2 - 10.1007/s00253-009-2174-6

DO - 10.1007/s00253-009-2174-6

M3 - Article

C2 - 19756586

AN - SCOPUS:76649085215

SN - 0175-7598

VL - 85

SP - 1551

EP - 1563

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

IS - 5

ER -

Inhibition of Candida albicans growth by brominated furanones

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this