Inhibition of biofilm formation and swarming of Bacillus subtilis by (5Z)-4-bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone

D. Ren, J. J. Sims, T. K. Wood

Research output: Contribution to journalArticlepeer-review

128 Scopus citations


Aims: (5Z)-4-Bromo-5-(bromomethylene)-3-butyl-2(5H)-furanone (furanone) of the marine alga Delisea pulchra was synthesized, and its inhibition of swarming motility and biofilm formation of Bacillus subtilis was investigated. Methods and Results: Furanone was found to inhibit both the growth of B. subtilis and its swarming motility in a concentration-dependent way. In addition, as shown by confocal scanning laser microscopy, furanone inhibited the biofilm formation of B. subtilis. At 40 lg ml)1, furanone decreased the biofilm thickness by 25%, decreased the number of water channels, and reduced the percentage of live cells by 63%. Conclusions, Significance and Impact of the Study: Natural furanone has potential for controlling the multicellular behaviour of Gram-positive bacteria.

Original languageEnglish (US)
Pages (from-to)293-299
Number of pages7
JournalLetters in Applied Microbiology
Issue number4
StatePublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology


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