TY - JOUR
T1 - Inheritance and linkage of allozymes and restriction fragment length polymorphisms in trembling aspen
AU - Furnier, G. R.
AU - Liu, Z.
AU - Furnier, G. R.
N1 - Funding Information:
From the Departments of Forest Resources (Liu and Furnier) and Plant Biology (Furnier), University of Minnesota, 1530 N. Cleveland Ave., St. Paul, MN 55108-6112. Dr. Liu is currently at the USDA/ARS/SAA, Southern Regional Plant Introduction Station, Griffin, Georgia. We thank Neil Anderson and Steve Lund for assistance in collecting flowering branches and performing crosses. Kathy Haiby and Tim O'Brien of the University of Minnesota North Central Experiment Station assisted in growing seedlings and collecting leaf tissue. Merlise Clyde assisted in the statistical analyses. Financial support was provided by the USDA Forest Service North Central Forest Experiment Station (cooperative research agreement no. 23-88-19), the Department of Forest Resources and College of Natural Resources at the University of Minnesota, and the Minnesota Agricultural Experiment Station. This is Minnesota Agricultural Experiment Station paper no. 19,831. Address correspondence to Glenn Furnier at the Department of Forest Resources.
PY - 1993/9
Y1 - 1993/9
N2 - Allozyme variants in leaf tissue of the parents and progeny of five full-sIb families of trembling aspen (Populus tremuloides; n = 19) were examined for nine enzyme systems. We consistently resolved allozymes of 14 loci, among which eight were polymorphic (Aco-1, Aco-2, Got-3, Per-1, Pgm-1, 6Pg-2, Pgi-2, and Skdh) and six were monomorphic (Got-1, Got-2, Mdh-1, Mdh-2, Pgl-1, and Sod). No significant deviation from the expected segregation ratios was observed for these allozyme loci. The results of joint two-locus segregation analyses indicated no linkage between 12 testable allozyme locus pairs. In one of these full-sib families of 93 progeny, we examined inheritance and linkage of four segregating allozyme loci and 75 RFLP loci corresponding to clones from an aspen Pst I genomic DNA library. None of the segregation ratios of the RFLP loci deviated significantly from those expected. We have constructed a linkage map that incorporates 54 RFLP and three allozyme loci comprising 14 linkage groups and covering 664 cM of the genome.
AB - Allozyme variants in leaf tissue of the parents and progeny of five full-sIb families of trembling aspen (Populus tremuloides; n = 19) were examined for nine enzyme systems. We consistently resolved allozymes of 14 loci, among which eight were polymorphic (Aco-1, Aco-2, Got-3, Per-1, Pgm-1, 6Pg-2, Pgi-2, and Skdh) and six were monomorphic (Got-1, Got-2, Mdh-1, Mdh-2, Pgl-1, and Sod). No significant deviation from the expected segregation ratios was observed for these allozyme loci. The results of joint two-locus segregation analyses indicated no linkage between 12 testable allozyme locus pairs. In one of these full-sib families of 93 progeny, we examined inheritance and linkage of four segregating allozyme loci and 75 RFLP loci corresponding to clones from an aspen Pst I genomic DNA library. None of the segregation ratios of the RFLP loci deviated significantly from those expected. We have constructed a linkage map that incorporates 54 RFLP and three allozyme loci comprising 14 linkage groups and covering 664 cM of the genome.
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U2 - 10.1093/oxfordjournals.jhered.a111365
DO - 10.1093/oxfordjournals.jhered.a111365
M3 - Article
AN - SCOPUS:0027522186
SN - 0022-1503
VL - 84
SP - 419
EP - 424
JO - Journal of Heredity
JF - Journal of Heredity
IS - 5
ER -