Inferring statistical properties of 3D cell geometry from 2D slices

Tristan A. Sharp, Matthias Merkel, Mary Elizabeth Manning, Andrea J. Liu

Research output: Contribution to journalArticle

Abstract

Although cell shape can reflect the mechanical and biochemical properties of the cell and its environment, quantification of 3D cell shapes within 3D tissues remains difficult, typically requiring digital reconstruction from a stack of 2D images. We investigate a simple alternative technique to extract information about the 3D shapes of cells in a tissue; this technique connects the ensemble of 3D shapes in the tissue with the distribution of 2D shapes observed in independent 2D slices. Using cell vertex model geometries, we find that the distribution of 2D shapes allows clear determination of the mean value of a 3D shape index. We analyze the errors that may arise in practice in the estimation of the mean 3D shape index from 2D imagery and find that typically only a few dozen cells in 2D imagery are required to reduce uncertainty below 2%. Even though we developed the method for isotropic animal tissues, we demonstrate it on an anisotropic plant tissue. This framework could also be naturally extended to estimate additional 3D geometric features and quantify their uncertainty in other materials.

Original languageEnglish (US)
Article numbere0209892
JournalPLoS One
Volume14
Issue number2
DOIs
StatePublished - Feb 1 2019

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Cell Shape
Tissue
Geometry
Imagery (Psychotherapy)
Uncertainty
cells
Tissue Distribution
uncertainty
Animals
geometry
animal tissues
plant tissues
methodology
tissues

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Inferring statistical properties of 3D cell geometry from 2D slices. / Sharp, Tristan A.; Merkel, Matthias; Manning, Mary Elizabeth; Liu, Andrea J.

In: PLoS One, Vol. 14, No. 2, e0209892, 01.02.2019.

Research output: Contribution to journalArticle

Sharp, Tristan A. ; Merkel, Matthias ; Manning, Mary Elizabeth ; Liu, Andrea J. / Inferring statistical properties of 3D cell geometry from 2D slices. In: PLoS One. 2019 ; Vol. 14, No. 2.
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