Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

Astrocytes produce numerous mediators under conditions of inflammation in the central nervous system. One such mediator is nitric oxide (NO) derived from nitric oxide synthase-2 (NOS-2), the high output, inducible NOS isoform. Expression of NOS-2 and production of NO can be stimulated in astrocyte cultures by combinations of cytokines and lipopolysaccharide, a gram-negative bacterial endotoxin. This chapter details methods to induce and analyze NOS-2 expression and NO production in astrocyte cultures.

Original languageEnglish (US)
Title of host publicationAstrocytes
Subtitle of host publicationMethods and Protocols
EditorsRichard Milner
Pages251-263
Number of pages13
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume814
ISSN (Print)1064-3745

Keywords

  • Astrocytes
  • Cytokines
  • Interferon-γ
  • Lipopolysaccharide
  • NO
  • Nitric oxide
  • Nitric oxide synthase-2
  • Transforming growth factor-β

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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