@inbook{03371023561e4625bff161134e90e832,
title = "Induction of nitric oxide synthase-2 expression and measurement of nitric oxide production in enriched primary cortical astrocyte cultures",
abstract = "Astrocytes produce numerous mediators under conditions of inflammation in the central nervous system. One such mediator is nitric oxide (NO) derived from nitric oxide synthase-2 (NOS-2), the high output, inducible NOS isoform. Expression of NOS-2 and production of NO can be stimulated in astrocyte cultures by combinations of cytokines and lipopolysaccharide, a gram-negative bacterial endotoxin. This chapter details methods to induce and analyze NOS-2 expression and NO production in astrocyte cultures.",
keywords = "Astrocytes, Cytokines, Interferon-γ, Lipopolysaccharide, NO, Nitric oxide, Nitric oxide synthase-2, Transforming growth factor-β",
author = "Hewett, {James A.} and Hewett, {Sandra J.}",
year = "2012",
doi = "10.1007/978-1-61779-452-0_17",
language = "English (US)",
isbn = "9781617794513",
series = "Methods in Molecular Biology",
pages = "251--263",
editor = "Richard Milner",
booktitle = "Astrocytes",
}