In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model

Seth Fillioe, Kyle K. Bishop, Alexander V.S. Jannini, John J.I. Kim, Ricky Mcdonough, Steve Ortiz, Jerry Goodisman, Julie Hasenwinkel, Charles M. Peterson, Joseph Chaiken

Research output: Contribution to journalArticle

Abstract

We report a small exploratory study of a methodology for real-time imaging of chemical and physical changes in spinal cords in the immediate aftermath of a localized contusive injury. One hundred separate experiments involving scanning NIR images, one-dimensional, two-dimensional (2-D), and point measurements, obtained in vivo, within a 3 × 7 mm field, on spinal cords surgically exposed between T9 and T10 revealed differences between injured and healthy cords. The collected raw data, i.e., elastic and inelastic emission from the laser probed tissues, combined via the PV[O]H algorithm, allow construction of five images over the first 5 h post injury. Within the larger study, a total of 13 rats were studied using 2-D images, i.e., injured and control. A single 830-nm laser (100-μm diameter round spot) was spatially line-scanned across the cord to reveal photobleaching effects and surface profiles possibly locating a near surface longitudinal artery/vein. In separate experiments, the laser was scanned in two dimensions across the exposed cord surface relative to the injury in a specific pattern to avoid uneven photobleaching of the imaged tissue. The 2-D scanning produced elastic and inelastic emission that allowed construction of PV[O]H images that had good fidelity with the visually observed surfaces and separate line scans and suggested differences between the volume fractions of fluid and turbidity of injured and healthy cord tissue.

Original languageEnglish (US)
Article number032007
JournalJournal of Biomedical Optics
Volume25
Issue number3
DOIs
StatePublished - Mar 1 2020

Fingerprint

spinal cord injuries
physiological responses
rats
Rats
Imaging techniques
spinal cord
Photobleaching
Tissue
lasers
scanning
Lasers
turbidity
arteries
veins
Scanning
Turbidity
methodology
fluids
Volume fraction
profiles

Keywords

  • hematocrit
  • imaging
  • in vivo
  • PV[O]H
  • spinal cord injury
  • turbidity

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model. / Fillioe, Seth; Bishop, Kyle K.; Jannini, Alexander V.S.; Kim, John J.I.; Mcdonough, Ricky; Ortiz, Steve; Goodisman, Jerry; Hasenwinkel, Julie; Peterson, Charles M.; Chaiken, Joseph.

In: Journal of Biomedical Optics, Vol. 25, No. 3, 032007, 01.03.2020.

Research output: Contribution to journalArticle

Fillioe, Seth ; Bishop, Kyle K. ; Jannini, Alexander V.S. ; Kim, John J.I. ; Mcdonough, Ricky ; Ortiz, Steve ; Goodisman, Jerry ; Hasenwinkel, Julie ; Peterson, Charles M. ; Chaiken, Joseph. / In vivo, noncontact, real-time, PV[O]H imaging of the immediate local physiological response to spinal cord injury in a rat model. In: Journal of Biomedical Optics. 2020 ; Vol. 25, No. 3.
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