In vivo, noncontact, real-Time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model

Seth Fillioe, Kyle Kelly Bishop, Alexander Vincent Struck Jannini, Jon Kim, Ricky McDonough, Steve Ortiz, Jerry Goodisman, Julie M Hasenwinkel, Joseph Chaiken

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We report a small study to test a methodology for real-Time probing of chemical and physical changes in spinal cords in the immediate aftermath of a localized contusive injury. Raman spectroscopy, optical profilimetry and scanning NIR autofluorescence images were obtained simultaneously in vivo, within a 3 x 7 mm field, on spinal cords that had been surgically exposed between T9 and T10. The collected data was used alone and/or combined in a unique algorithm. A total of six rats were studied in two N=3 groups i.e. Injured and Control. A single 830 nm laser (100 μm round spot) was either 1) spatially scanned across the cord or 2) held at a specified location relative to the injury for a longer period of time to improve signal to noise in the Raman spectra. Line scans reveal photobleaching effects and surface profiles possibly allowing identification of the anterior median longitudinal artery. Analysis of the Raman spectra suggest that the tissues were equally hypoxic for both the control and injured animals i.e. a possible artifact of anesthesia and surgery. On the other hand, only injured cords display Raman features possibly indicating that extensive, localized protein phosphorylation occurs in minutes following spinal cord trauma.

Original languageEnglish (US)
Title of host publicationOptical Biopsy XVI
Subtitle of host publicationToward Real-Time Spectroscopic Imaging and Diagnosis
PublisherSPIE
Volume10489
ISBN (Electronic)9781510614635
DOIs
StatePublished - Jan 1 2018
EventOptical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2018 - San Francisco, United States
Duration: Jan 30 2018Jan 31 2018

Other

OtherOptical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2018
CountryUnited States
CitySan Francisco
Period1/30/181/31/18

Fingerprint

spinal cord injuries
physiological responses
spinal cord
Raman Spectrum Analysis
Spinal Cord Injuries
rats
Rats
Spinal Cord
Photobleaching
Wounds and Injuries
Artifacts
Raman spectra
anesthesia
Noise
phosphorylation
Lasers
Anesthesia
Arteries
Phosphorylation
Raman scattering

Keywords

  • Fluorescence
  • Hematocrit
  • Imaging
  • In vivo
  • Inflammation
  • Noncontact
  • Raman spectroscopy
  • Spinal cord Injury

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Fillioe, S., Bishop, K. K., Jannini, A. V. S., Kim, J., McDonough, R., Ortiz, S., ... Chaiken, J. (2018). In vivo, noncontact, real-Time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model. In Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis (Vol. 10489). [104890B] SPIE. https://doi.org/10.1117/12.2290500

In vivo, noncontact, real-Time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model. / Fillioe, Seth; Bishop, Kyle Kelly; Jannini, Alexander Vincent Struck; Kim, Jon; McDonough, Ricky; Ortiz, Steve; Goodisman, Jerry; Hasenwinkel, Julie M; Chaiken, Joseph.

Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10489 SPIE, 2018. 104890B.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fillioe, S, Bishop, KK, Jannini, AVS, Kim, J, McDonough, R, Ortiz, S, Goodisman, J, Hasenwinkel, JM & Chaiken, J 2018, In vivo, noncontact, real-Time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model. in Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. vol. 10489, 104890B, SPIE, Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis 2018, San Francisco, United States, 1/30/18. https://doi.org/10.1117/12.2290500
Fillioe S, Bishop KK, Jannini AVS, Kim J, McDonough R, Ortiz S et al. In vivo, noncontact, real-Time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model. In Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10489. SPIE. 2018. 104890B https://doi.org/10.1117/12.2290500
Fillioe, Seth ; Bishop, Kyle Kelly ; Jannini, Alexander Vincent Struck ; Kim, Jon ; McDonough, Ricky ; Ortiz, Steve ; Goodisman, Jerry ; Hasenwinkel, Julie M ; Chaiken, Joseph. / In vivo, noncontact, real-Time, optical and spectroscopic assessment of the immediate local physiological response to spinal cord injury in a rat model. Optical Biopsy XVI: Toward Real-Time Spectroscopic Imaging and Diagnosis. Vol. 10489 SPIE, 2018.
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