In vitro evaluation of decellularized ECM-derived surgical scaffold biomaterials

Xiao Luo, Katherine M. Kulig, Eric B. Finkelstein, Margaret F. Nicholson, Xiang Hong Liu, Scott M. Goldman, Joseph P. Vacanti, Brian E. Grottkau, Irina Pomerantseva, Cathryn A. Sundback, Craig M. Neville

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Decellularized extracellular matrix (ECM) biomaterials are increasingly used in regenerative medicine for abdominal tissue repair. Emerging ECM biomaterials with greater compliance target surgical procedures like breast and craniofacial reconstruction to enhance aesthetic outcome. Clinical studies report improved outcomes with newly designed ECM scaffolds, but their comparative biological characteristics have received less attention. In this study, we investigated scaffolds derived from dermis (AlloDerm Regenerative Tissue Matrix), small intestinal submucosa (Surgisis 4-layer Tissue Graft and OASIS Wound Matrix), and mesothelium (Meso BioMatrix Surgical Mesh and Veritas Collagen Matrix) and evaluated biological properties that modulate cellular responses and recruitment. An assay panel was utilized to assess the ECM scaffold effects upon cells. Results of the material-conditioned media study demonstrated Meso BioMatrix and OASIS best supported cell proliferation. Meso BioMatrix promoted the greatest migration and chemotaxis signaling, followed by Veritas and OASIS; OASIS had superior suppression of cell apoptosis. The direct adhesion assay indicated that AlloDerm, Meso BioMatrix, Surgisis, and Veritas had sidedness that affected cell-material interactions. In the chick chorioallantoic membrane assay, Meso BioMatrix and OASIS best supported cell infiltration. Among tested materials, Meso BioMatrix and OASIS demonstrated characteristics that facilitate scaffold incorporation, making them promising choices for many clinical applications.

Original languageEnglish (US)
Pages (from-to)585-593
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume105
Issue number3
DOIs
StatePublished - Apr 1 2017

Keywords

  • cell adhesion
  • cell proliferation
  • extracellular matrix
  • in vitro
  • scaffolds

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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  • Cite this

    Luo, X., Kulig, K. M., Finkelstein, E. B., Nicholson, M. F., Liu, X. H., Goldman, S. M., Vacanti, J. P., Grottkau, B. E., Pomerantseva, I., Sundback, C. A., & Neville, C. M. (2017). In vitro evaluation of decellularized ECM-derived surgical scaffold biomaterials. Journal of Biomedical Materials Research - Part B Applied Biomaterials, 105(3), 585-593. https://doi.org/10.1002/jbm.b.33572