Improved molecular tools for sugar cane biotechnology

Mark Kinkema, Jason Geijskes, Paulo deLucca, Anthony Palupe, Kylie Shand, Heather D. Coleman, Anthony Brinin, Brett Williams, Manuel Sainz, James L. Dale

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Sugar cane is a major source of food and fuel worldwide. Biotechnology has the potential to improve economically-important traits in sugar cane as well as diversify sugar cane beyond traditional applications such as sucrose production. High levels of transgene expression are key to the success of improving crops through biotechnology. Here we describe new molecular tools that both expand and improve gene expression capabilities in sugar cane. We have identified promoters that can be used to drive high levels of gene expression in the leaf and stem of transgenic sugar cane. One of these promoters, derived from the Cestrum yellow leaf curling virus, drives levels of constitutive transgene expression that are significantly higher than those achieved by the historical benchmark maize polyubiquitin-1 (Zm-Ubi1) promoter. A second promoter, the maize phosphonenolpyruvate carboxylate promoter, was found to be a strong, leaf-preferred promoter that enables levels of expression comparable to Zm-Ubi1 in this organ. Transgene expression was increased approximately 50-fold by gene modification, which included optimising the codon usage of the coding sequence to better suit sugar cane. We also describe a novel dual transcriptional enhancer that increased gene expression from different promoters, boosting expression from Zm-Ubi1 over eightfold. These molecular tools will be extremely valuable for the improvement of sugar cane through biotechnology.

Original languageEnglish (US)
Pages (from-to)497-508
Number of pages12
JournalPlant Molecular Biology
Volume84
Issue number4-5
DOIs
StatePublished - Mar 1 2014

Keywords

  • Agrobacterium-mediated transformation
  • Enhancer
  • Gene expression
  • Genetic engineering
  • Promoter
  • Transgenic sugar cane

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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  • Cite this

    Kinkema, M., Geijskes, J., deLucca, P., Palupe, A., Shand, K., Coleman, H. D., Brinin, A., Williams, B., Sainz, M., & Dale, J. L. (2014). Improved molecular tools for sugar cane biotechnology. Plant Molecular Biology, 84(4-5), 497-508. https://doi.org/10.1007/s11103-013-0147-8