Imaging the early zebrafish embryo centrosomes following injection of small-molecule inhibitors to understand spindle formation

Abrar A. Aljiboury, Amra Mujcic, Thomas Cammerino, Lindsay I. Rathbun, Heidi Hehnly

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

During the earliest division stages, zebrafish embryos have large cells that divide rapidly and synchronously to create a cellular layer on top of the yolk. Here, we describe a protocol for monitoring spindle dynamics during these early embryonic divisions. We outline techniques for injecting zebrafish embryos with small-molecule inhibitors toward polo-like kinases, preparing and mounting embryos for three-dimensional imaging using confocal microscopy. These techniques are used to understand how the early zebrafish embryo's centrosome constructs the mitotic spindle. For complete details on the use and execution of this protocol, please refer to Rathbun et al. (2020).

Original languageEnglish (US)
Article number100293
JournalSTAR Protocols
Volume2
Issue number1
DOIs
StatePublished - Mar 19 2021

Keywords

  • Cell biology
  • Microscopy
  • Model organisms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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