Identification, annotation and expression analysis of 29 Rho GTPase genes from channel catfish (Ictalurus punctatus) after bacterial infections

Suxu Tan, Jun Yao, Tao Zhou, Shikai Liu, Zihao Yuan, Changxu Tian, Qi Li, Zhanjian "John" Liu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The Rho family GTPases are a group of small monomeric G proteins, which are molecular switches in signaling pathways. They have been known to regulate a diverse range of cellular processes including actin cytoskeleton rearrangement and microtubule dynamics. In particular, their participations in immune responses are also significant. However, little information of the Rho GTPases is available in teleost including channel catfish, an economically important species and one of the best teleost models forimmunological research. In this study, Rho GTPase genes were identified from channel catfish and well annotated by phylogenetic and syntenic analyses. Their expression profiles were determined in channel catfish healthy tissues and infected tissues. Altogether seven Rho GTPase genes were significantly regulated after bacterial infection, with six genes in the gill after Flavobacterium columnare challenge and two genes in the intestine in response to Edwardsiella ictaluri. All the differentially expressed genes were up-regulated soon after bacterial infection. Different expression patterns between the two experiments were observed, which may be attributed to tissue-specific regulation or pathogen-specific regulation. These results suggested that Rho GTPases play important roles in immune responses to bacterial pathogens, setting a foundation for future investigation on Rho GTPases.

Original languageEnglish (US)
Pages (from-to)445-451
Number of pages7
JournalDevelopmental and Comparative Immunology
Volume67
DOIs
StatePublished - Feb 1 2017
Externally publishedYes

Fingerprint

Ictaluridae
rho GTP-Binding Proteins
Bacterial Infections
Genes
Monomeric GTP-Binding Proteins
Edwardsiella ictaluri
Flavobacterium
Actin Cytoskeleton
Microtubules
Intestines
Research

Keywords

  • Catfish
  • Genome
  • Immune response
  • Infection
  • Rho GTPases

ASJC Scopus subject areas

  • Immunology
  • Developmental Biology

Cite this

Identification, annotation and expression analysis of 29 Rho GTPase genes from channel catfish (Ictalurus punctatus) after bacterial infections. / Tan, Suxu; Yao, Jun; Zhou, Tao; Liu, Shikai; Yuan, Zihao; Tian, Changxu; Li, Qi; Liu, Zhanjian "John".

In: Developmental and Comparative Immunology, Vol. 67, 01.02.2017, p. 445-451.

Research output: Contribution to journalArticle

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