Hypersensitive response-like lesions 1 codes for AtPPT1 and regulates accumulation of ROS and defense against bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana

Aditya Dutta, Samuel H P Chan, Noel T. Pauli, Ramesh Raina

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aims: Plants employ both basal and resistance gene (R gene)-mediated defenses in response to pathogens. Reactive oxygen species (ROS) are widely reported to play a central role in both basal and R gene-mediated defense; however, the nature of ROS has been less well established for basal defense. In addition, spatial distribution of redox moieties and mechanisms of plant responses during basal defense are poorly understood. We investigated redox signaling in Arabidopsis thaliana in response to virulent bacterial pathogen, focusing on the role of the mitochondria in balancing energy demands against generation of physiologically relevant ROS. Results: Positional cloning of an Arabidopsis lesion mimic mutant identified a polyprenyl transferase involved in the biosynthesis of Coenzyme Q10 (CoQ), which leads to novel insights into physiological ROS levels and their role in basal resistance. Gain- and loss-of-function studies identified Coenzyme Q10 redox state to be a key determinant of ROS levels. These Coenzyme Q10 redox state-mediated ROS levels had a direct bearing on both response against pathogen and ability to thrive in high oxidative stress environments. Innovation: We demonstrate that Coenzyme Q10 redox state generates an ROS threshold for a successful basal resistance response. Perturbation of the Coenzyme Q10 redox state has the potential to disrupt plant defense responses against bacterial pathogens. Conclusions: Coenzyme Q10 redox state is a key regulator of Arabidopsis basal resistance against bacterial pathogens. Antioxid. Redox Signal. 22, 785-796.

Original languageEnglish (US)
Pages (from-to)785-796
Number of pages12
JournalAntioxidants and Redox Signaling
Volume22
Issue number9
DOIs
StatePublished - Mar 20 2015

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coenzyme Q10
Pseudomonas syringae
Pathogens
Arabidopsis
Oxidation-Reduction
Reactive Oxygen Species
Genes
Mitochondria
Oxidative stress
Cloning
Biosynthesis
Transferases
Spatial distribution
Organism Cloning
Oxidative Stress
Innovation

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Physiology
  • Clinical Biochemistry

Cite this

Hypersensitive response-like lesions 1 codes for AtPPT1 and regulates accumulation of ROS and defense against bacterial pathogen Pseudomonas syringae in Arabidopsis thaliana. / Dutta, Aditya; Chan, Samuel H P; Pauli, Noel T.; Raina, Ramesh.

In: Antioxidants and Redox Signaling, Vol. 22, No. 9, 20.03.2015, p. 785-796.

Research output: Contribution to journalArticle

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