Hydrogen-bonding effects and 13 of the DNA double helix

Philip N. Borer, Steven R. Laplante, Nibo Zanatta, George C. Levy

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


13C-nmr chemical shifts of the nucleotides in DNA are sensitive to hydrogen bonding, especially for three of the carbons immediately bonded to exocyclic oxygen or nitrogen atoms acting as H-bond acceptors or donors. GuoC2, GuoC6 and ThdC4 are strongly deshielded (about 1 ppm) upon Watson-Crick pairing in oligodeoxynucleotide duplexes, regardless of the base sequence. Deshielding at these sites may be useful to distinguish bases involved in Watson-Crick pairs from unpaired bases.

Original languageEnglish (US)
Pages (from-to)2323-2332
Number of pages10
JournalNucleic acids research
Issue number5
StatePublished - Mar 11 1988

ASJC Scopus subject areas

  • Genetics


Dive into the research topics of 'Hydrogen-bonding effects and 13 of the DNA double helix'. Together they form a unique fingerprint.

Cite this