Hydrogen-bonding effects and 13 of the DNA double helix

Philip N. Borer, Steven R. Laplante, Nibo Zanatta, George C. Levy

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

13C-nmr chemical shifts of the nucleotides in DNA are sensitive to hydrogen bonding, especially for three of the carbons immediately bonded to exocyclic oxygen or nitrogen atoms acting as H-bond acceptors or donors. GuoC2, GuoC6 and ThdC4 are strongly deshielded (about 1 ppm) upon Watson-Crick pairing in oligodeoxynucleotide duplexes, regardless of the base sequence. Deshielding at these sites may be useful to distinguish bases involved in Watson-Crick pairs from unpaired bases.

Original languageEnglish (US)
Pages (from-to)2323-2332
Number of pages10
JournalNucleic acids research
Volume16
Issue number5
DOIs
StatePublished - Mar 11 1988

ASJC Scopus subject areas

  • Genetics

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    Borer, P. N., Laplante, S. R., Zanatta, N., & Levy, G. C. (1988). Hydrogen-bonding effects and 13 of the DNA double helix. Nucleic acids research, 16(5), 2323-2332. https://doi.org/10.1093/nar/16.5.2323