Human Nek7-interactor RGS2 is required for mitotic spindle organization

Edmarcia Elisa De Souza, Heidi Hehnly, Arina Marina Perez, Gabriela Vaz Meirelles, Juliana Helena Costa Smetana, Stephen Doxsey, Jörg Kobarg

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The mitotic spindle apparatus is composed of microtubule (MT) networks attached to kinetochores organized from 2 centrosomes (a.k.a. spindle poles). In addition to this central spindle apparatus, astral MTs assemble at the mitotic spindle pole and attach to the cell cortex to ensure appropriate spindle orientation. We propose that cell cycle-related kinase, Nek7, and its novel interacting protein RGS2, are involved in mitosis regulation and spindle formation. We found that RGS2 localizes to the mitotic spindle in a Nek7-dependent manner, and along with Nek7 contributes to spindle morphology and mitotic spindle pole integrity. RGS2-depletion leads to a mitotic-delay and severe defects in the chromosomes alignment and congression. Importantly, RGS2 or Nek7 depletion or even overexpression of wild-type or kinase-dead Nek7, reduced γ-tubulin from the mitotic spindle poles. In addition to causing a mitotic delay, RGS2 depletion induced mitotic spindle misorientation coinciding with astral MT-reduction. We propose that these phenotypes directly contribute to a failure in mitotic spindle alignment to the substratum. In conclusion, we suggest a molecular mechanism whereupon Nek7 and RGS2 may act cooperatively to ensure proper mitotic spindle organization.

Original languageEnglish (US)
Pages (from-to)656-667
Number of pages12
JournalCell Cycle
Issue number4
StatePublished - Feb 15 2015
Externally publishedYes


  • Cell division
  • Mitotic spindle
  • Mitotic spindle orientation
  • Nek7
  • RGS2

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology


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